Product Description: histone-lysine N-methyltransferase SET2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.43 (Transferred entry: 2.1.1.354)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000785 | chromatin |
| Component | GO:0005694 | chromosome |
| Component | GO:0034399 | nuclear periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0003682 | chromatin binding |
| Function | GO:0046975 | histone methyltransferase activity (H3-K36 specific) |
| Function | GO:0018024 | histone-lysine N-methyltransferase activity |
| Function | GO:0031493 | nucleosomal histone binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0010452 | histone H3-K36 methylation |
| Process | GO:0097198 | histone H3-K36 trimethylation |
| Process | GO:0006355 | regulation of transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.78 | 0.36 |
| Trophozoite | expressed | 0.73 | 0.62 |
| Schizont | expressed | 0.39 | 0.79 |
| Gametocyte | expressed | 0.82 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1322100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.151 | #Insertions: 10
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE95
"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.02) with 4IJ8 (Global RMSD=5.15)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.43 | histone-lysine N-methyltransferase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 125 | 69 | 164 |
| disruptive | 114 | 360 | 231 | 408 |
| missense | 68 | 298 | 196 | 327 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 42 | 236 | 116 | 168 |
| disruptive | 205 | 753 | 328 | 703 |
| missense | 126 | 591 | 269 | 443 |
PlasmoDB Total SNPs: 432
Non-coding: 149 | Synonymous: 167 | Nonsynonymous: 116 | Stop Codon: 0
Protein Length: 2548 | Molecular Weight (kDa): 300.714
UniProt ID(s): Q8IE95
PDB ID(s): None
Isoelectric Point: 7.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001214 | SET domain |
| InterPro | IPR001965 | Zinc finger, PHD-type |
| PFam | PF00856 | SET domain |
| Superfamily | SSF82199 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18299133 | Histone lysine methyltransferases and demethylases in Plasmodium falciparum. | Cui L, Fan Q, Cui L, Miao J | 10.1016/j.ijpara.2008.01.002 |
| 19765590 | Potential epigenetic regulatory proteins localise to distinct nuclearsub-compartments in Plasmodium falciparum. | Volz J, Carvalho TG, ..., Cowman AF | 10.1016/j.ijpara.2009.09.002 |
| 23823717 | PfSETvs methylation of histone H3K36 represses virulence genes in Plasmodiumfalciparum. | Jiang L, Mu J, ..., Miller LH | 10.1038/nature12361 |
| 24391504 | Recruitment of PfSET2 by RNA polymerase II to variant antigen encoding locicontributes to antigenic variation in P. falciparum. | Ukaegbu UE, Kishore SP, ..., Deitsch KW | 10.1371/journal.ppat.1003854 |
| 34268135 | The Transcription Factor PfAP2-O Influences Virulence Gene Transcription andSexual Development in Plasmodium falciparum. | Cubillos EFG, Prata IO, ..., Wunderlich G | https://pubmed.ncbi.nlm.nih.gov/34268135/ |
| 34389510 | Plasmodium falciparum SET2 domain is allosterically regulated by its PHD-likedomain to methylate at H3K36. | Sethumadhavan DV, Govindaraju G, Jabeena CA, Rajavelu A | 10.1016/j.bbagrm.2021.194744 |
| 37796660 | Gcn5- and Bre1-mediated Set2 degradation promotes chronological aging ofSaccharomyces cerevisiae | Li YM, Mei YC, ..., Du HN | 10.1016/j.celrep.2023.113186 |