PF3D7_1322500 (DHHC5)

Genome location: Pf3D7_13_v3:952,855..955,994(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC5

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: 2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Component	GO:0016020	membrane
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Function	GO:0008270	zinc ion binding
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.05	0.98
Trophozoite	expressed	0.41	0.76
Schizont	expressed	0.94	0.49
Gametocyte	expressed	0.57	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1322500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.966 | MFS: -2.787 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1337800)

Modification: Tagged | RMgm-899

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE90

"Best" AlphaFill ligand hit: BU2 (1,3-butanediol, Local RMSD=0.18) with 2RFM (Global RMSD=1.47)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_165769

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	33	21	54
disruptive	6	55	32	123
missense	6	55	31	110

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	68	32	58
disruptive	20	131	76	142
missense	14	119	70	120

PlasmoDB Total SNPs: 116

Non-coding: 77 | Synonymous: 23 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 676 | Molecular Weight (kDa): 80.837

UniProt ID(s): Q8IE90

PDB ID(s): None

Isoelectric Point: 9.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
InterPro	IPR002110	Ankyrin repeat
InterPro	IPR020683	Ankyrin repeat-containing domain
InterPro	IPR036770	Ankyrin repeat-containing domain superfamily
PFam	PF01529	Palmitoyltransferase, DHHC domain
PFam	PF12796	Ankyrin repeat-containing domain
Superfamily	SSF48403	Ankyrin repeat-containing domain superfamily