Product Description: beta-hydroxyacyl-ACP dehydratase
SignalP Peptide: MRFLIIHIAVIVLPFVLM
# Transmembrane Domains: 1
EC Numbers: 4.2.1.59 (3-hydroxyacyl-[acyl-carrier-protein] dehydratase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.04 | 0.98 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1323000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.148 | MFS: -2.709 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1338200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1338200)
Modification: Disrupted | RMgm-3582
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6T4
"Best" AlphaFill ligand hit: QUE (quercetin, Local RMSD=0.02) with 3CF8 (Global RMSD=2.30)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.59 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase | p-chloromercuribenzoate |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q965D7 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase | Plasmodium falciparum | OMA, OrthoMCL, OrthoMCL BLAST | CHEMBL1269411Gallocatechin 3-O-gallateacs.jmedchem.1c00409_ST.600med.21724, Compound 4... |
| O25928 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ | Helicobacter pylori | OrthoMCL, OrthoMCL BLAST | carbohydrazide derivative, 3hcarbohydrazide derivative, 3icarbohydrazide derivative, 3jcarbohydrazide derivative, 3k... |
| A0A0E3A638 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ | OrthoMCL BLAST | alpha-mangostinJuglone (6a) | |
| B1JQH1 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ [1-175] | OrthoMCL BLAST | alpha-mangostinJuglone (6a) |
Protein Length: 230 | Molecular Weight (kDa): 26.211
PDB ID(s): None
Isoelectric Point: 10.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010084 | Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ |
| InterPro | IPR013114 | Beta-hydroxydecanoyl thiol ester dehydrase, FabA/FabZ |
| InterPro | IPR029069 | HotDog domain superfamily |
| PFam | PF07977 | Beta-hydroxydecanoyl thiol ester dehydrase, FabA/FabZ |
| Superfamily | SSF54637 | HotDog domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 13 | 10 | 15 |
| disruptive | 3 | 25 | 17 | 45 |
| missense | 3 | 24 | 17 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 24 | 8 | 16 |
| disruptive | 7 | 65 | 33 | 49 |
| missense | 5 | 60 | 30 | 41 |
PlasmoDB Total SNPs: 64
Non-coding: 49 | Synonymous: 7 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9770490 | Nuclear-encoded proteins target to the plastid in Toxoplasma gondii andPlasmodium falciparum. | Waller RF, Keeling PJ, ..., McFadden GI | 10.1073/pnas.95.21.12352 |
| 21843645 | Structural basis for the functional and inhibitory mechanisms ofbeta-hydroxyacyl-acyl carrier protein dehydratase (FabZ) of Plasmodium falciparum. | Maity K, Venkata BS, ..., Suguna K | 10.1016/j.jsb.2011.07.018 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34531837 | The Role of Type II Fatty Acid Synthesis Enzymes FabZ, ODSCI, and ODSCII in thePathogenesis of Toxoplasma gondii Infection | Xu XP, Elsheikha HM, ..., Zhu XQ | 10.3389/fmicb.2021.703059 |
| 33260377 | Regulation of the First Committed Step in Lipopolysaccharide BiosynthesisCatalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease | Biernacka D, Gorzelak P, Klein G, Raina S | 10.3390/ijms21239088 |
| 36464488 | LapB (YciM) orchestrates protein-protein interactions at the interface oflipopolysaccharide and phospholipid biosynthesis | Moller AM, Bruckner S, ..., Narberhaus F | 10.1111/mmi.15005 |