PF3D7_1323200

Genome location: Pf3D7_13_v3:967,817..969,229(-)

Genome classification: Core

Function and Localization

Product Description: V-type proton ATPase subunit G, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2);3.6.3.6 (Transferred entry: 7.1.2.1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0020020	food vacuole
Component	GO:0005634	nucleus
Component	GO:0016471	vacuolar proton-transporting V-type ATPase complex
Component	GO:0000221	vacuolar proton-transporting V-type ATPase, V1 domain
Function	GO:0008553	P-type proton-exporting transporter activity
Process	GO:0007035	vacuolar acidification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.46	0.79
Trophozoite	expressed	0.84	0.55
Schizont	expressed	0.38	0.75
Gametocyte	expressed	0.77	0.58

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1323200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.354 | MFS: -3.572 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1338400)

Relative Growth Rate: 0.20 ± 0.17
Confidence: 4.96

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE84

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.3.14	H+-transporting two-sector ATPase	No BRENDA inhibitors
3.6.3.6	H+-exporting ATPase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101449

Most Similar Human Ortholog: A0A7I2V4K4

TM-align score: 0.96 | RMSD: 0.63

Seq Identity: 0.09 | Length: 71 / 123

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136888	ATPase H+ transporting V1 subunit G1
ENSG00000206445	ATPase H+ transporting V1 subunit G2
ENSG00000213760	ATPase H+ transporting V1 subunit G2
ENSG00000226850	ATPase H+ transporting V1 subunit G2
ENSG00000227587	ATPase H+ transporting V1 subunit G2
ENSG00000230900	ATPase H+ transporting V1 subunit G2
ENSG00000234668	ATPase H+ transporting V1 subunit G2
ENSG00000234920	ATPase H+ transporting V1 subunit G2
ENSG00000254870	ATP6V1G2-DDX39B readthrough (NMD candidate)
ENSG00000254899	ATPase H+ transporting lysosomal 13kDa V1 subunit G isoform 2 (ATP6V1G2) and HLA-B associated transcript 1 (BAT1) readth

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	0	0	1
missense	0	0	0	0

Any inclusion in genotype call

variant type	rare	doubleton	singleton
synonymous	0	0	0
disruptive	1	1	1
missense	0	0	0

PlasmoDB Total SNPs: 86

Non-coding: 81 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 123 | Molecular Weight (kDa): 14.192

UniProt ID(s): Q8IE84

PDB ID(s): None

Isoelectric Point: 8.36

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005124	Vacuolar (H+)-ATPase G subunit
PFam	PF03179	Vacuolar (H+)-ATPase G subunit
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
21136929	Food vacuole proteome of the malarial parasite Plasmodium falciparum.	Lamarque M, Tastet C, ..., Dubremetz JF	10.1002/prca.200700112
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g