PF3D7_1323700 (GAPM1)

Genome location: Pf3D7_13_v3:981,651..984,211(+)

Genome classification: Core

Function and Localization

Product Description: glideosome associated protein with multiple membrane spans 1

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Component	GO:0070258	inner membrane pellicle complex
Component	GO:0032991	protein-containing complex
Function	GO:0005515	protein binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.12	0.93
Schizont	expressed	1.37	0.37
Gametocyte	expressed	0.47	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1323700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.254 | MFS: -2.63 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1338900)

Relative Growth Rate: 0.11 ± 0.16
Confidence: 5.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE80

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_130918

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	16	8	18
disruptive	2	4	1	13
missense	2	4	1	12

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	29	10	26
disruptive	3	17	17	68
missense	2	11	11	46

PlasmoDB Total SNPs: 185

Non-coding: 178 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 303 | Molecular Weight (kDa): 33.98

UniProt ID(s): Q8IE80

PDB ID(s): None

Isoelectric Point: 4.62

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR021691	Protein of unknown function DUF3273
PFam	PF11677	Protein of unknown function DUF3273
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
19465059	A complex of three related membrane proteins is conserved on malarial merozoites.	Rayavara K, Rajapandi T, ..., Desai SA	10.1016/j.molbiopara.2009.05.006
19561073	A novel family of Apicomplexan glideosome-associated proteins with an innermembrane-anchoring role.	Bullen HE, Tonkin CJ, ..., Gilson PR	10.1074/jbc.M109.036772
20037583	Transcriptional profiling of growth perturbations of the human malaria parasitePlasmodium falciparum.	Hu G, Cabrera A, ..., Bozdech Z	10.1038/nbt.1597
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606
28985225	Disrupting assembly of the inner membrane complex blocks Plasmodium falciparumsexual stage development.	Parkyn Schneider M, Liu B, ..., Dixon MWA	10.1371/journal.ppat.1006659
29138437	Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the innermembrane complex and is required for Plasmodium parasite development	Saini E, Zeeshan M, ..., Tewari R	10.1038/s41598-017-15781-z