PF3D7_1324500

Genome location: Pf3D7_13_v3:1,022,857..1,028,042(-)

Genome classification: Core

Function and Localization

Product Description: DEAD box helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Component	GO:0005634	nucleus
Function	GO:0003678	DNA helicase activity
Function	GO:0004386	helicase activity
Process	GO:0032508	DNA duplex unwinding
Process	GO:0034085	establishment of sister chromatid cohesion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.10	0.95
Trophozoite	expressed	0.76	0.59
Schizont	possibly expressed	0.18	0.89
Gametocyte	possibly expressed	0.59	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1324500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.143 | MFS: -3.241 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE72

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9BX63	Fanconi anemia group J protein		OrthoDB	Telomestatin (TMS)Telomestatin (TMS)Telomestatin (TMS)TMPyP4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102316

Most Similar Human Ortholog: Q96FC9

TM-align score: 0.66 | RMSD: 5.08

Seq Identity: 0.22 | Length: 738 / 1099

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000013573	DEAD/H-box helicase 11

Protein Information

Protein Length: 1099 | Molecular Weight (kDa): 130.702

UniProt ID(s): Q8IE72

PDB ID(s): None

Isoelectric Point: 8.7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006554	Helicase-like, DEXD box c2 type
InterPro	IPR006555	ATP-dependent helicase, C-terminal
InterPro	IPR010614	DEAD2
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR028331	ATP-dependent RNA helicase CHL1/DDX11
PFam	PF06733	DEAD2
PFam	PF13307	ATP-dependent helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	49	33	73
disruptive	12	90	71	142
missense	12	90	67	131

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	101	45	76
disruptive	41	215	125	228
missense	29	187	111	181

PlasmoDB Total SNPs: 353

Non-coding: 309 | Synonymous: 31 | Nonsynonymous: 13 | Stop Codon: 0