PF3D7_1325200

Genome location: Pf3D7_13_v3:1,047,544..1,049,934(-)

Genome classification: Core

Function and Localization

Product Description: lactate dehydrogenase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.1.1.27 (L-lactate dehydrogenase);1.1.1.37 (Malate dehydrogenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005829	cytosol
Function	GO:0004459	L-lactate dehydrogenase activity
Function	GO:0030060	L-malate dehydrogenase activity
Process	GO:0006089	lactate metabolic process
Process	GO:0006090	pyruvate metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.01	1.00
Trophozoite	expressed	0.01	0.99
Schizont	expressed	0.01	0.99
Gametocyte	expressed	0.35	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1325200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.613 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1340400)

Relative Growth Rate: 1.02 ± 0.10
Confidence: 5.98

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1340400)

Modification: Disrupted | RMgm-3594

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE66

"Best" AlphaFill ligand hit: NAI (nadh, Local RMSD=0.18) with 4OKN (Global RMSD=3.63)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.1.1.27	L-lactate dehydrogenase	BRENDA SOAP query unsuccessful
1.1.1.37	malate dehydrogenase	ATPADPCoAAMPNEMNAD+NADHEDTAcAMPDMSONADP+HgCl2...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P16125	L-lactate dehydrogenase B chain		OMA	Gossypol (C9)
P40926	Malate dehydrogenase, mitochondrial	Homo sapiens	OrthoDB	CHEMBL427092CHEMBL3359141CHEMBL3359147CHEMBL427092...
Q6VVP7	L-lactate dehydrogenase	Plasmodium falciparum	OrthoMCL BLAST	Gossypol (C9)Oxamic acid derivative, 5Oxamic acid derivative, 7
B9UDZ5	L-lactate dehydrogenase	Plasmodium falciparum	OrthoMCL BLAST	Gossypol (C9)
Q27743	L-lactate dehydrogenase	Plasmodium falciparum	OrthoMCL BLAST	Gossypol (C9)Oxamic acid derivative, 5Oxamic acid derivative, 6Oxamic acid derivative, 7...

Orthology Information

Ortholog Group (OrthoMCL): OG6_200216

No human ortholog(s)

Protein Information

Protein Length: 334 | Molecular Weight (kDa): 37.008

UniProt ID(s): A0A097PB34, Q0PJ46, Q8IE66

PDB ID(s): None

Isoelectric Point: 6.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001236	Lactate/malate dehydrogenase, N-terminal
InterPro	IPR001557	L-lactate/malate dehydrogenase
InterPro	IPR011275	Malate dehydrogenase, type 3
InterPro	IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal
InterPro	IPR022383	Lactate/malate dehydrogenase, C-terminal
InterPro	IPR036291	NAD(P)-binding domain superfamily
PFam	PF00056	Lactate/malate dehydrogenase, N-terminal
PFam	PF02866	Lactate/malate dehydrogenase, C-terminal
Superfamily	SSF51735	NAD(P)-binding domain superfamily
Superfamily	SSF56327	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	15	12	28
disruptive	0	1	0	6
missense	0	1	0	3

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	39	26	19
disruptive	1	17	19	46
missense	0	7	16	35

PlasmoDB Total SNPs: 140

Non-coding: 119 | Synonymous: 21 | Nonsynonymous: 0 | Stop Codon: 0