PF3D7_1326400

Genome location: Pf3D7_13_v3:1,095,046..1,099,223(-)

Genome classification: Core

Function and Localization

Product Description: translation initiation factor eIF-2B subunit gamma, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005851	eukaryotic translation initiation factor 2B complex
Process	GO:0006413	translational initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.24	0.88
Trophozoite	expressed	0.38	0.77
Schizont	expressed	0.05	0.97
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1326400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.186 | MFS: -2.835 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1341600)

Relative Growth Rate: 0.06 ± 0.09
Confidence: 6.24

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K977

"Best" AlphaFill ligand hit: CTP (cytidine-5'-triphosphate, Local RMSD=4.82) with 7ECD (Global RMSD=15.70)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102755

Most Similar Human Ortholog: A0A0D9SEN0

TM-align score: 0.82 | RMSD: 2.19

Seq Identity: 0.17 | Length: 126 / 473

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000070785	eukaryotic translation initiation factor 2B subunit gamma

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	27	17	32
disruptive	1	21	11	48
missense	1	21	10	41

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	57	22	40
disruptive	8	65	48	128
missense	2	49	37	80

PlasmoDB Total SNPs: 286

Non-coding: 262 | Synonymous: 22 | Nonsynonymous: 1 | Stop Codon: 1

Protein Information

Protein Length: 473 | Molecular Weight (kDa): 55.042

UniProt ID(s): A0A5K1K977

PDB ID(s): None

Isoelectric Point: 7.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR029044	Nucleotide-diphospho-sugar transferases
PFam	N/A	N/A
Superfamily	SSF53448	Nucleotide-diphospho-sugar transferases