Genome location: Pf3D7_13_v3:1,142,949..1,144,084(+)
Genome classification: Core
Product Description: ribonuclease P protein subunit RPR2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.26.5 (Ribonuclease P)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005655 | nucleolar ribonuclease P complex |
| Component | GO:0030677 | ribonuclease P complex |
| Function | GO:0004526 | ribonuclease P activity |
| Process | GO:0008033 | tRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.34 | 0.84 |
| Trophozoite | no data | 0.40 | 0.77 |
| Schizont | no data | 0.04 | 0.98 |
| Gametocyte | no data | 0.17 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1327200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.143 | MFS: -2.899 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1342400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE34
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.26.5 | ribonuclease P | ATPRNAEDTANH4+tRNASO42-NeoR5NeoG6NeoK6retinolneaminemat-tRNA... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103265
Most Similar Human Ortholog: Q9H633
TM-align score: 0.59 | RMSD: 1.98
Seq Identity: 0.23 | Length: 101 / 252
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000241370 | ribonuclease P/MRP subunit p21 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 6 | 5 | 13 |
| disruptive | 8 | 33 | 18 | 46 |
| missense | 6 | 33 | 17 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 17 | 9 | 15 |
| disruptive | 15 | 78 | 31 | 51 |
| missense | 11 | 72 | 26 | 38 |
PlasmoDB Total SNPs: 48
Non-coding: 26 | Synonymous: 9 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 252 | Molecular Weight (kDa): 29.94
UniProt ID(s): Q8IE34
PDB ID(s): None
Isoelectric Point: 9.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007175 | RNAse P, Rpr2/Rpp21 subunit |
| PFam | PF04032 | RNAse P, Rpr2/Rpp21 subunit |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25273134 | Enhancement of RNA annealing and strand displacement found in archaealribonuclease P proteins is conserved in Escherichia coli protein C5 and yeastprotein Rpr2 | Furutani T, Hazeyama K, ..., Kimura M | 10.1080/09168451.2014.925780 |
| 15637077 | Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals anew unique protein component | Salinas K, Wierzbicki S, Zhou L, Schmitt ME | 10.1074/jbc.M409568200 |
| 37074161 | Proteins Rpr2 and Pop3 increase the activity and thermal stability of yeast RNaseP | Perederina A, Berezin I, Krasilnikov AS | 10.1080/15476286.2023.2201110 |