Genome location: Pf3D7_13_v3:1,158,691..1,160,403(+)
Genome classification: Core
Product Description: nicotinamide/nicotinic acid mononucleotide adenylyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.18 (Nicotinate-nucleotide adenylyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0000309 | nicotinamide-nucleotide adenylyltransferase activity |
| Function | GO:0004515 | nicotinate-nucleotide adenylyltransferase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.05 | 0.97 |
| Trophozoite | no data | 0.02 | 0.99 |
| Schizont | no data | 0.09 | 0.95 |
| Gametocyte | no data | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1327600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.754 | MFS: -2.892 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1342800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1342800)
Modification: Disrupted | RMgm-3604
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE38
"Best" AlphaFill ligand hit: ADE (adenine, Local RMSD=0.16) with 8AII (Global RMSD=3.15)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.18 | nicotinate-nucleotide adenylyltransferase | NMNNAD+K891-0166Tithonia diversifolia oilnicotinic acid adenine dinucleotide2-phenyl-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamideN-[4-(4-iodophenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamideN'-[(E)-biphenyl-4-ylmethylidene]-5-(4-bromophenyl)-4-oxopentanehydrazideN'-[(E)-anthracen-9-ylmethylidene]-5-(4-bromophenyl)-4-oxopentanehydrazideN'-[(E)-anthracen-9-ylmethylidene]-5-(3-bromophenyl)-4-oxopentanehydrazideN'-[(E)-anthracen-9-ylmethylidene]-5-(2-bromophenyl)-4-oxopentanehydrazideN'-[(E)-biphenyl-4-ylmethylidene]-5-(4-fluorophenyl)-4-oxopentanehydrazide... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0A752 | Nicotinate-nucleotide adenylyltransferase | Escherichia coli | OrthoMCL, OrthoMCL BLAST | NadD inhibitor 1_02NadD inhibitor 1_02 |
| P65502 | Probable nicotinate-nucleotide adenylyltransferase | OrthoMCL BLAST | MLS002638004MLS000079801MLS001076665MLS002693528... | |
| C3L5T6 | Probable nicotinate-nucleotide adenylyltransferase | Bacillus anthracis | OrthoMCL BLAST | NadD inhibitor 3_05NadD inhibitor 1_02Compound ID 5824Compound ID 5{1}... |
Ortholog Group (OrthoMCL): OG6_100907
Most Similar Human Ortholog: A0A2R8YFG2
TM-align score: 0.81 | RMSD: 2.53
Seq Identity: 0.18 | Length: 192 / 204
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163864 | nicotinamide nucleotide adenylyltransferase 3 |
| ENSG00000173614 | nicotinamide nucleotide adenylyltransferase 1 |
Protein Length: 204 | Molecular Weight (kDa): 24.282
UniProt ID(s): Q8IE38
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004821 | Cytidyltransferase-like domain |
| PFam | PF01467 | Cytidyltransferase-like domain |
| Superfamily | SSF52374 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 4 | 6 | 9 |
| disruptive | 1 | 3 | 1 | 5 |
| missense | 1 | 3 | 1 | 5 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 14 | 7 | 13 |
| disruptive | 2 | 7 | 13 | 35 |
| missense | 1 | 4 | 11 | 24 |
PlasmoDB Total SNPs: 106
Non-coding: 94 | Synonymous: 10 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 24747974 | Targeting NAD+ metabolism in the human malaria parasite Plasmodium falciparum. | O'Hara JK, Kerwin LJ, ..., Llinas M | 10.1371/journal.pone.0094061 |
| 27984041 | Structural and Functional Characterization of Plasmodium falciparum NicotinicAcid Mononucleotide Adenylyltransferase. | Bathke J, Fritz-Wolf K, ..., Becker K | 10.1016/j.jmb.2016.10.023 |
| 29642910 | Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinatemononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages. | Nieto CA, Sanchez LM, ..., Ramirez MH | 10.1186/s12936-018-2307-4 |