PF3D7_1327800

Genome location: Pf3D7_13_v3:1,162,463..1,165,039(+)

Genome classification: Core

Function and Localization

Product Description: ribose-phosphate pyrophosphokinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.6.1 (Ribose-phosphate diphosphokinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005739	mitochondrion
Component	GO:0005634	nucleus
Component	GO:0002189	ribose phosphate diphosphokinase complex
Function	GO:0004749	ribose phosphate diphosphokinase activity
Process	GO:0006015	5-phosphoribose 1-diphosphate biosynthetic process
Process	GO:0000105	histidine biosynthetic process
Process	GO:0009116	nucleoside metabolic process
Process	GO:0009165	nucleotide biosynthetic process
Process	GO:0006164	purine nucleotide biosynthetic process
Process	GO:0000162	tryptophan biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.44	0.79
Trophozoite	expressed	0.48	0.72
Schizont	expressed	0.07	0.95
Gametocyte	expressed	0.23	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1327800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.992 | MFS: -1.359 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1343000)

Relative Growth Rate: 0.88 ± 0.11
Confidence: 5.84

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1343000)

Modification: Disrupted | RMgm-3605

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE40

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.13) with 6NFE (Global RMSD=8.69)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.6.1	ribose-phosphate diphosphokinase	ATPADPFADUDPAMPGTPGDPCTPUTPUMPGSHGMP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P60891	Ribose-phosphate pyrophosphokinase 1	Homo sapiens	OrthoDB	US10981917, Example 1US10981917, Example 2US10981917, Example 4US10981917, Example 5...

Orthology Information

Ortholog Group (OrthoMCL): OG6_159065

No human ortholog(s)

Protein Information

Protein Length: 560 | Molecular Weight (kDa): 64.019

UniProt ID(s): Q8IE40

PDB ID(s): None

Isoelectric Point: 7.89

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000836	Phosphoribosyltransferase domain
InterPro	IPR005946	Ribose-phosphate pyrophosphokinase
InterPro	IPR029057	Phosphoribosyltransferase-like
InterPro	IPR029099	Ribose-phosphate pyrophosphokinase, N-terminal domain
PFam	PF13793	Ribose-phosphate pyrophosphokinase, N-terminal domain
PFam	PF14572	Ribose-phosphate pyrophosphokinase
Superfamily	SSF53271	Phosphoribosyltransferase-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	22	6	18
disruptive	5	16	13	41
missense	5	16	11	34

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	33	11	27
disruptive	9	55	21	87
missense	6	44	15	51

PlasmoDB Total SNPs: 53

Non-coding: 31 | Synonymous: 16 | Nonsynonymous: 6 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21