Product Description: proteasome subunit beta type-7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Component | GO:0044310 | osmiophilic body |
| Component | GO:0005839 | proteasome core complex |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0010498 | proteasomal protein catabolic process |
| Process | GO:0010499 | proteasomal ubiquitin-independent protein catabolic process |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.78 | 0.58 |
| Schizont | expressed | 0.36 | 0.78 |
| Gametocyte | expressed | 0.46 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1328100
Resistome Missense Mutations: C72Y, C72F, A90E
Resistome Compounds with Missense Mutations: WLW
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.244 | MFS: -2.338 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1343300)
Modification: Other | RMgm-453
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6T3
"Best" AlphaFill ligand hit: GQK ((2~{s})-3-(4-methoxyphenyl)-~{n}-[(2~{s},3~{r})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{s})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide, Local RMSD=0.04) with 6HTC (Global RMSD=1.05)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P40306 | Proteasome subunit beta type-10 | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL3237875CHEMBL3319481CHEMBL3237875CHEMBL3319481... | |
| O35955 | Proteasome subunit beta type-10 | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL4452892Kzr-616CHEMBL4452892CHEMBL4465456... | |
| Q99436 | Proteasome subunit beta type-7 | HOGENOM, OrthoDB | CHEMBL3237866CHEMBL3237864BORTEZOMIBCHEMBL3237869... |
Ortholog Group (OrthoMCL): OG6_101382
Most Similar Human Ortholog: P40306
TM-align score: 0.93 | RMSD: 1.81
Seq Identity: 0.46 | Length: 264 / 270
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136930 | proteasome 20S subunit beta 7 |
| ENSG00000205220 | proteasome 20S subunit beta 10 |
Protein Length: 270 | Molecular Weight (kDa): 29.962
UniProt ID(s): Q8I6T3
PDB ID(s): 5FMG, 6MUV, 6MUW, 6MUX, 7LXT, 7LXU
Isoelectric Point: 7.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000243 | Peptidase T1A, proteasome beta-subunit |
| InterPro | IPR001353 | Proteasome, subunit alpha/beta |
| InterPro | IPR016050 | Proteasome beta-type subunit, conserved site |
| InterPro | IPR029055 | Nucleophile aminohydrolases, N-terminal |
| InterPro | IPR035216 | Proteasome subunit beta 7 |
| PFam | PF00227 | Proteasome, subunit alpha/beta |
| Superfamily | SSF56235 | Nucleophile aminohydrolases, N-terminal |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 77
Non-coding: 69 | Synonymous: 8 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 27432909 | Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodiumfalciparum Osmiophilic Bodies in Malaria Parasite Transmission. | Suarez-Cortes P, Sharma V, ..., Alano P | 10.1074/mcp.M116.060681 |
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |