PF3D7_1329000

Genome location: Pf3D7_13_v3:1,217,710..1,226,469(-)

Genome classification: Core

Function and Localization

Product Description: DNA-directed RNA polymerase III subunit RPC1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005666	RNA polymerase III complex
Function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
Process	GO:0006383	transcription by RNA polymerase III

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.73	0.66
Trophozoite	not expressed	0.68	0.63
Schizont	not expressed	0.24	0.85
Gametocyte	not expressed	0.50	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1329000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.953 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1344000)

Relative Growth Rate: 0.21 ± 0.20
Confidence: 4.63

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE49

"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.20) with 6OW3 (Global RMSD=4.16)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.6	DNA-directed RNA polymerase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O95602	DNA-directed RNA polymerase I subunit RPA1		OrthoDB	CHEMBL3289403CHEMBL3289408CHEMBL3289409CHEMBL3289410...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100210

Most Similar Human Ortholog: O14802

TM-align score: 0.93 | RMSD: 2.61

Seq Identity: 0.44 | Length: 1336 / 2356

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000148606	RNA polymerase III subunit A
ENSG00000181222	RNA polymerase II subunit A
ENSG00000284832	RNA polymerase II subunit A

Protein Information

Protein Length: 2356 | Molecular Weight (kDa): 274.831

UniProt ID(s): P27625, Q8IE49

PDB ID(s): None

Isoelectric Point: 7.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000722	RNA polymerase, alpha subunit
InterPro	IPR006592	RNA polymerase, N-terminal
InterPro	IPR007066	RNA polymerase Rpb1, domain 3
InterPro	IPR007080	RNA polymerase Rpb1, domain 1
InterPro	IPR007081	RNA polymerase Rpb1, domain 5
InterPro	IPR007083	RNA polymerase Rpb1, domain 4
InterPro	IPR035697	DNA-directed RNA polymerase III subunit RPC1, N-terminal
PFam	PF00623	RNA polymerase, alpha subunit
PFam	PF04983	RNA polymerase Rpb1, domain 3
PFam	PF04997	RNA polymerase Rpb1, domain 1
PFam	PF04998	RNA polymerase Rpb1, domain 5
PFam	PF05000	RNA polymerase Rpb1, domain 4
Superfamily	SSF64484	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	101	67	144
disruptive	57	196	101	289
missense	33	140	85	228

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	243	80	142
disruptive	112	393	190	384
missense	56	278	149	278

PlasmoDB Total SNPs: 389

Non-coding: 169 | Synonymous: 136 | Nonsynonymous: 84 | Stop Codon: 0