Genome location: Pf3D7_13_v3:1,238,815..1,241,964(-)
Genome classification: Core
Product Description: chromatin assembly factor 1 subunit B, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0003682 | chromatin binding |
| Function | GO:0042393 | histone binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0031497 | chromatin assembly |
| Process | GO:0006333 | chromatin assembly or disassembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.11 | 0.94 |
| Trophozoite | possibly expressed | 0.66 | 0.62 |
| Schizont | expressed | 0.36 | 0.78 |
| Gametocyte | not expressed | 0.55 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1329300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.16 | MFS: -2.736 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1344300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE52
"Best" AlphaFill ligand hit: ALQ (2-methyl-propionic acid, Local RMSD=1.20) with 4GM3 (Global RMSD=3.18)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 11 | 40 |
| disruptive | 1 | 13 | 12 | 50 |
| missense | 1 | 13 | 12 | 42 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 37 | 18 | 45 |
| disruptive | 6 | 37 | 31 | 91 |
| missense | 3 | 29 | 26 | 66 |
PlasmoDB Total SNPs: 151
Non-coding: 134 | Synonymous: 15 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 582 | Molecular Weight (kDa): 66.512
UniProt ID(s): Q8IE52
PDB ID(s): None
Isoelectric Point: 5.26
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR019775 | WD40 repeat, conserved site |
| InterPro | IPR022052 | Histone-binding protein RBBP4, N-terminal |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| PFam | PF00400 | WD40 repeat |
| PFam | PF12265 | Histone-binding protein RBBP4, N-terminal |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29183721 | Role of Chromatin assembly factor 1 in DNA replication of Plasmodium falciparum. | Gupta MK, Agarawal M, ..., Dhar SK | 10.1016/j.bbrc.2017.11.131 |
| 38132148 | Independent and Complementary Functions of Caf1b and Hir1 for Chromatin Assembly in Tetrahymena thermophila | Hao H, Ren C, ..., Wang W | 10.3390/cells12242828 |
| 22941664 | Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei | Alsford S, Horn D | 10.1093/nar/gks813 |