Genome location: Pf3D7_13_v3:1,242,635..1,247,339(+)
Genome classification: Core
Product Description: AMP deaminase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.5.4.6 (AMP deaminase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Function | GO:0003876 | AMP deaminase activity |
| Process | GO:0006196 | AMP catabolic process |
| Process | GO:0046033 | AMP metabolic process |
| Process | GO:0006188 | IMP biosynthetic process |
| Process | GO:0006163 | purine nucleotide metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.21 | 0.86 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1329400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.995 | MFS: -1.778 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE53
"Best" AlphaFill ligand hit: NOS (inosine, Local RMSD=0.06) with 6II7 (Global RMSD=1.68)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.5.4.6 | AMP deaminase | ATPADPNH3GTPUTPKClIMPEDTAdATPTLCKMgSO43'-AMP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O09178 | AMP deaminase 3 | OrthoDB | 6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3) | |
| P23109 | AMP deaminase 1 | OrthoDB | 6-[4-({[(1R)-1-(naphthalen-1-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 1)6-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 2)6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)4-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]-2-methoxybenzoic acid (Compound 4)... | |
| Q02356 | AMP deaminase 2 | OrthoDB | 6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)4-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]-2-methoxybenzoic acid (Compound 4) | |
| O80452 | AMP deaminase | Arabidopsis thaliana | OrthoDB, OrthoMCL | CHEMBL1160282 |
| P10759 | AMP deaminase 1 | OrthoDB | 6-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 2)6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)4-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]-2-methoxybenzoic acid (Compound 4) | |
| Q01433 | AMP deaminase 2 | OrthoDB | 6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)CHEMBL290887CHEMBL433444CHEMBL36045... | |
| Q3V1D3 | AMP deaminase 1 | OrthoDB | 6-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 2)6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)4-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]-2-methoxybenzoic acid (Compound 4) | |
| Q01432 | AMP deaminase 3 | OrthoDB | 6-[4-({[(1R)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 2)6-[4-({[(1S)-1-(isoquinolin-8-yl)ethyl]amino}methyl)phenyl]pyridine-3-carboxylic acid (Compound 3)CHEMBL290684CHEMBL37273... |
Ortholog Group (OrthoMCL): OG6_100681
Most Similar Human Ortholog: A0A804H2H4
TM-align score: 0.89 | RMSD: 2.56
Seq Identity: 0.42 | Length: 587 / 693
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116337 | adenosine monophosphate deaminase 2 |
| ENSG00000116748 | adenosine monophosphate deaminase 1 |
| ENSG00000133805 | adenosine monophosphate deaminase 3 |
Protein Length: 693 | Molecular Weight (kDa): 81.772
UniProt ID(s): Q8IE53
PDB ID(s): None
Isoelectric Point: 8.51
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001365 | Adenosine/AMP deaminase domain |
| InterPro | IPR006329 | AMP deaminase |
| InterPro | IPR032466 | Metal-dependent hydrolase |
| PFam | PF00962 | Adenosine/AMP deaminase domain |
| Superfamily | SSF51556 | Metal-dependent hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 37 | 24 | 49 |
| disruptive | 2 | 27 | 27 | 56 |
| missense | 2 | 27 | 24 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 84 | 36 | 55 |
| disruptive | 5 | 77 | 66 | 130 |
| missense | 4 | 61 | 56 | 99 |
PlasmoDB Total SNPs: 344
Non-coding: 322 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 31128470 | Nucleocytoplasmic O-glycosylation in protists. | West CM, Kim HW | 10.1016/j.sbi.2019.03.031 |
| 31132185 | Biochemical and physiological investigations on adenosine 5' monophosphatedeaminase from Plasmodium spp. | Nagappa LK, Singh D, ..., Balaram H | 10.1111/mmi.14313 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |