PF3D7_1330300

Genome location: Pf3D7_13_v3:1,275,888..1,278,531(-)

Genome classification: Core

Function and Localization

Product Description: DnaJ protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Process	GO:0009408	response to heat
Process	GO:0006986	response to unfolded protein

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.14	0.93
Trophozoite	possibly expressed	0.72	0.60
Schizont	possibly expressed	0.18	0.88
Gametocyte	expressed	0.52	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1330300

Resistome Missense Mutations: V251F, D252Y, D252V

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.673 | #Insertions: 2

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1345300)

Relative Growth Rate: 0.17 ± 0.21
Confidence: 4.54

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5E9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_121818

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	17	12	24
disruptive	12	18	22	43
missense	12	17	22	40

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	39	24	21
disruptive	20	53	47	106
missense	17	44	40	76

PlasmoDB Total SNPs: 167

Non-coding: 151 | Synonymous: 6 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 331 | Molecular Weight (kDa): 39.574

UniProt ID(s): C0H5E9

PDB ID(s): None

Isoelectric Point: 10.3

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001623	DnaJ domain
InterPro	IPR018253	DnaJ domain, conserved site
InterPro	IPR036869	Chaperone J-domain superfamily
PFam	PF00226	DnaJ domain
Superfamily	SSF46565	Chaperone J-domain superfamily