PF3D7_1330500

Genome location: Pf3D7_13_v3:1,284,735..1,287,844(+)

Genome classification: Core

Function and Localization

Product Description: CTLH domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0034657	GID complex
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.61	0.65
Gametocyte	expressed	1.37	0.36

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1330500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.012 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE21

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103231

Most Similar Human Ortholog: B4DQT1

TM-align score: 0.78 | RMSD: 3.64

Seq Identity: 0.27 | Length: 333 / 725

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000090316	macrophage erythroblast attacher, E3 ubiquitin ligase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	25	19	35
disruptive	70	104	56	128
missense	21	57	42	85

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	49	27	52
disruptive	105	189	89	184
missense	37	110	66	107

PlasmoDB Total SNPs: 303

Non-coding: 147 | Synonymous: 85 | Nonsynonymous: 70 | Stop Codon: 1

Protein Information

Protein Length: 725 | Molecular Weight (kDa): 86.166

UniProt ID(s): Q8IE21

PDB ID(s): None

Isoelectric Point: 5.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006595	CTLH, C-terminal LisH motif
InterPro	IPR024964	CTLH/CRA C-terminal to LisH motif domain
InterPro	IPR027714	Protein Fyv10/Macrophage erythroblast attacher
PFam	PF10607	CTLH/CRA C-terminal to LisH motif domain
Superfamily	SSF57850	N/A