Product Description: elongation factor Tu, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0003746 | translation elongation factor activity |
| Process | GO:0070125 | mitochondrial translational elongation |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.32 | 0.79 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.37 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1330600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.779 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1345600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE20
"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.08) with 2PMD (Global RMSD=8.63)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100780
Most Similar Human Ortholog: P49411
TM-align score: 0.88 | RMSD: 1.86
Seq Identity: 0.51 | Length: 411 / 505
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000178952 | Tu translation elongation factor, mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 22 | 16 | 27 |
| disruptive | 4 | 20 | 14 | 37 |
| missense | 4 | 19 | 14 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 55 | 18 | 30 |
| disruptive | 8 | 58 | 25 | 84 |
| missense | 8 | 47 | 21 | 70 |
PlasmoDB Total SNPs: 37
Non-coding: 27 | Synonymous: 5 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 505 | Molecular Weight (kDa): 57.632
UniProt ID(s): Q8IE20
PDB ID(s): None
Isoelectric Point: 9.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR004160 | Translation elongation factor EFTu/EF1A, C-terminal |
| InterPro | IPR004161 | Translation elongation factor EFTu-like, domain 2 |
| InterPro | IPR004541 | Translation elongation factor EFTu/EF1A, bacterial/organelle |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR009001 | Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031157 | Tr-type G domain, conserved site |
| InterPro | IPR033720 | Elongation factor Tu, domain 2 |
| InterPro | IPR041709 | Elongation factor Tu (EF-Tu), GTP-binding domain |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF03143 | Translation elongation factor EFTu/EF1A, C-terminal |
| PFam | PF03144 | Translation elongation factor EFTu-like, domain 2 |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF50465 | Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 21163263 | Interaction of apicoplast-encoded elongation factor (EF) EF-Tu withnuclear-encoded EF-Ts mediates translation in the Plasmodiumfalciparum plastid. | Biswas S, Lim EE, ..., Habib S | 10.1016/j.ijpara.2010.11.003 |
| 21741312 | Protein translation in Plasmodium parasites. | Jackson KE, Habib S, ..., Ralph SA | 10.1016/j.pt.2011.05.005 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |