PF3D7_1331100 (POLQ)

Genome location: Pf3D7_13_v3:1,299,417..1,305,427(-)

Genome classification: Core

Function and Localization

Product Description: DNA polymerase theta, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0003724	RNA helicase activity
Process	GO:0006401	RNA catabolic process
Process	GO:0000460	maturation of 5.8S rRNA

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.24	0.88
Trophozoite	expressed	0.40	0.77
Schizont	expressed	1.22	0.46
Gametocyte	expressed	0.68	0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1331100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.018 | #Insertions: 11

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1346000)

Relative Growth Rate: 1.02 ± 0.06
Confidence: 7.11

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1346000)

Modification: Disrupted | RMgm-3619

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5F0

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.15) with 6IEG (Global RMSD=8.09)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O75417	DNA polymerase theta		OrthoDB	CHEMBL5185248CHEMBL5190448CHEMBL5175626CHEMBL5188740...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101025

Most Similar Human Ortholog: O75417

TM-align score: 0.38 | RMSD: 6.86

Seq Identity: 0.21 | Length: 585 / 1244

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000051341	DNA polymerase theta
ENSG00000163312	helicase, POLQ like

Protein Information

Protein Length: 1244 | Molecular Weight (kDa): 147.386

UniProt ID(s): C0H5F0

PDB ID(s): None

Isoelectric Point: 8.25

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	64	35	76
disruptive	36	81	49	134
missense	17	66	42	104

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	114	50	90
disruptive	57	205	92	267
missense	27	155	74	180

PlasmoDB Total SNPs: 371

Non-coding: 253 | Synonymous: 76 | Nonsynonymous: 42 | Stop Codon: 0