PF3D7_1331600 (DEH)

Genome location: Pf3D7_13_v3:1,316,161..1,317,997(-)

Genome classification: Core

Function and Localization

Product Description: 3-hydroxyacyl-CoA dehydratase DEH

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.1.3.48 (Protein-tyrosine-phosphatase);4.2.1.134 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Component	GO:0030176	integral component of endoplasmic reticulum membrane
Function	GO:0018812	3-hydroxyacyl-CoA dehydratase activity
Process	GO:0030497	fatty acid elongation
Process	GO:0030148	sphingolipid biosynthetic process
Process	GO:0042761	very long-chain fatty acid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.97
Trophozoite	expressed	0.36	0.77
Schizont	expressed	0.26	0.83
Gametocyte	expressed	0.47	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1331600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.145 | MFS: -2.772 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1346500)

Relative Growth Rate: 1.01 ± 0.06
Confidence: 7.13

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1346500)

Modification: Disrupted | RMgm-4873

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5F1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.48	protein-tyrosine-phosphatase	BRENDA SOAP query unsuccessful
4.2.1.134	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase	flufenacet

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101538

Most Similar Human Ortholog: Q6Y1H2

TM-align score: 0.84 | RMSD: 2.39

Seq Identity: 0.28 | Length: 215 / 228

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000165996	3-hydroxyacyl-CoA dehydratase 1
ENSG00000206527	3-hydroxyacyl-CoA dehydratase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	17	7	12
disruptive	1	9	12	28
missense	1	9	10	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	30	10	18
disruptive	4	34	25	46
missense	2	26	20	34

PlasmoDB Total SNPs: 129

Non-coding: 126 | Synonymous: 1 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 228 | Molecular Weight (kDa): 26.387

UniProt ID(s): C0H5F1

PDB ID(s): None

Isoelectric Point: 9.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007482	Protein-tyrosine phosphatase-like, PTPLA
PFam	PF04387	Protein-tyrosine phosphatase-like, PTPLA
Superfamily	N/A	N/A