PF3D7_1331700

Genome location: Pf3D7_13_v3:1,318,841..1,323,158(-)

Genome classification: Core

Function and Localization

Product Description: glutamine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.18 (Glutamine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0004819	glutamine-tRNA ligase activity
Process	GO:0006425	glutaminyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.50	0.76
Trophozoite	expressed	0.77	0.58
Schizont	expressed	0.33	0.78
Gametocyte	expressed	0.72	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1331700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.205 | MFS: -2.692 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1346600)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE10

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.26) with 1ZJW (Global RMSD=1.70)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.18	glutamine-tRNA ligase	tRNAGlu-AMSTheaninealbizziineL-Glutamic acidS-CarbamoylserineS-Carbamoylcysteineglutaminol adenylate4-Methyleneglutaminep-hydroxymercuribenzoateGlutamic acid 4-hydroxamate5,5'-dithiobis(2-nitrobenzoate)...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P47897	Glutamine--tRNA ligase	Homo sapiens	OrthoDB	CHEMBL609187CHEMBL609187CHEMBL608302CHEMBL609496

Orthology Information

Ortholog Group (OrthoMCL): OG6_101072

Most Similar Human Ortholog: H7C0R3

TM-align score: 0.94 | RMSD: 1.64

Seq Identity: 0.37 | Length: 252 / 918

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000172053	glutaminyl-tRNA synthetase 1

Protein Information

Protein Length: 918 | Molecular Weight (kDa): 108.532

UniProt ID(s): Q8IE10

PDB ID(s): None

Isoelectric Point: 8.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000924	Glutamyl/glutaminyl-tRNA synthetase
InterPro	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site
InterPro	IPR004514	Glutamine-tRNA synthetase
InterPro	IPR011035	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily
InterPro	IPR020058	Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
InterPro	IPR020059	Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain
PFam	PF00749	Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
PFam	PF03950	Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain
Superfamily	SSF50715	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily
Superfamily	SSF52374

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	46	27	47
disruptive	18	75	43	102
missense	9	55	36	82

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	99	37	52
disruptive	31	144	66	179
missense	16	108	56	123

PlasmoDB Total SNPs: 187

Non-coding: 119 | Synonymous: 49 | Nonsynonymous: 17 | Stop Codon: 2

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108