PF3D7_1332400

Genome location: Pf3D7_13_v3:1,336,921..1,340,631(-)

Genome classification: Core

Function and Localization

Product Description: nucleotidyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.59	0.73
Trophozoite	expressed	0.73	0.60
Schizont	expressed	0.22	0.87
Gametocyte	expressed	0.77	0.58

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1332400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.956 | MFS: -0.942 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1347200)

Relative Growth Rate: 0.71 ± 0.08
Confidence: 6.42

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1347200)

Modification: Disrupted | RMgm-3628

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8X2

"Best" AlphaFill ligand hit: UTP (uridine 5'-triphosphate, Local RMSD=1.02) with 6L8K (Global RMSD=9.17)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.7	DNA-directed DNA polymerase	KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101092

Most Similar Human Ortholog: D6RJD0

TM-align score: 0.82 | RMSD: 0.63

Seq Identity: 0.30 | Length: 27 / 858

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000112941	terminal nucleotidyltransferase 4A
ENSG00000121274	terminal nucleotidyltransferase 4B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	43	28	62
disruptive	23	97	66	144
missense	15	89	57	136

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	92	33	55
disruptive	44	216	99	184
missense	29	177	92	146

PlasmoDB Total SNPs: 143

Non-coding: 76 | Synonymous: 39 | Nonsynonymous: 28 | Stop Codon: 0

Protein Information

Protein Length: 858 | Molecular Weight (kDa): 102.029

UniProt ID(s): A0A5K1K8X2

PDB ID(s): None

Isoelectric Point: 9.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002058	PAP/25A-associated
InterPro	IPR002934	Polymerase, nucleotidyl transferase domain
InterPro	IPR043519	Nucleotidyltransferase superfamily
PFam	PF01909	Polymerase, nucleotidyl transferase domain
PFam	PF03828	PAP/25A-associated
Superfamily	SSF81301	Nucleotidyltransferase superfamily
Superfamily	SSF81631