PF3D7_1332500

Genome location: Pf3D7_13_v3:1,340,768..1,341,856(+)

Genome classification: Core

Function and Localization

Product Description: SAM-dependent RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.220 (tRNA (adenine(58)-N(1))-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0035241	protein-arginine omega-N monomethyltransferase activity
Process	GO:0018216	peptidyl-arginine methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.04	0.98
Schizont	expressed	0.01	0.99
Gametocyte	expressed	0.04	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1332500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.998 | MFS: -2.591 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE03

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.66) with 5H5F (Global RMSD=3.84)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.220	tRNA (adenine58-N1)-methyltransferase

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_109089

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	11	8	15
disruptive	1	9	2	14
missense	1	9	2	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	25	11	17
disruptive	2	17	11	29
missense	2	16	9	21

PlasmoDB Total SNPs: 85

Non-coding: 79 | Synonymous: 4 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 218 | Molecular Weight (kDa): 25.489

UniProt ID(s): Q8IE03

PDB ID(s): None

Isoelectric Point: 4.46

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007364	RNA methyltransferase TK0422/Sfm1
PFam	PF04252	RNA methyltransferase TK0422/Sfm1
Superfamily	N/A	N/A