Genome location: Pf3D7_13_v3:1,340,764..1,344,123(-)
Genome classification: Core
Product Description: DNA-(apurinic or apyrimidinic site) lyase 1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 4.2.99.18 (DNA-(apurinic or apyrimidinic site) lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0003906 | DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Function | GO:0030145 | manganese ion binding |
| Function | GO:0016791 | phosphatase activity |
| Function | GO:0008081 | phosphoric diester hydrolase activity |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0006284 | base-excision repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 1.16 | 0.44 |
| Schizont | expressed | 1.11 | 0.47 |
| Gametocyte | expressed | 0.71 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1332600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.941 | MFS: -2.627 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1347400)
Modification: Tagged | RMgm-4985
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE02
"Best" AlphaFill ligand hit: BCN (bicine, Local RMSD=0.32) with 2X7W (Global RMSD=1.50)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.99.18 | DNA-(apurinic or apyrimidinic site) lyase | ATPAMPKClCMPUMPP53EDTAdCMPdTMPdAMPdGMPATCA... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 19 | 8 | 46 |
| disruptive | 30 | 65 | 43 | 98 |
| missense | 13 | 54 | 35 | 80 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 42 | 27 | 32 |
| disruptive | 56 | 133 | 79 | 116 |
| missense | 21 | 108 | 65 | 94 |
PlasmoDB Total SNPs: 2
Non-coding: 2 | Synonymous: 0 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 597 | Molecular Weight (kDa): 69.722
UniProt ID(s): Q8IE02, Q964Q4, Q9BMG7
PDB ID(s): None
Isoelectric Point: 9.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001719 | AP endonuclease 2 |
| InterPro | IPR013022 | Xylose isomerase-like, TIM barrel domain |
| InterPro | IPR018246 | AP endonuclease 2, zinc binding site |
| InterPro | IPR036237 | Xylose isomerase-like superfamily |
| PFam | PF01261 | Xylose isomerase-like, TIM barrel domain |
| Superfamily | SSF51658 | Xylose isomerase-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 31386260 | Plasmodium falciparum Apn1 homolog is a mitochondrial base excision repairprotein with restricted enzymatic functions. | Tiwari A, Kuldeep J, Siddiqi MI, Habib S | 10.1111/febs.15032 |
| 32075635 | Identification, molecular characterization and expression of aminopeptidase N-1(APN-1) from Anopheles stephensi in SF9 cell line as a candidate molecule fordeveloping a vaccine that interrupt malaria transmission | Dadgar Pakdel J, Zakeri S, Raz A, Dinparast Djadid N | 10.1186/s12936-020-03154-3 |
| 36430884 | Kinetic Features of 3'-5'-Exonuclease Activity of Apurinic/ApyrimidinicEndonuclease Apn2 from Saccharomyces cerevisiae | Kuznetsova AA, Gavrilova AA, ..., Kuznetsov NA | 10.3390/ijms232214404 |
| 33108581 | Astaxanthin protects oxidative stress mediated DNA damage and enhances longevityin Saccharomyces cerevisiae | Sudharshan SJ, Dyavaiah M | 10.1007/s10522-020-09904-9 |
| 35809390 | C. elegans ribosomal protein S3 protects against H(2)O(2)-induced DNA damage andsuppresses spontaneous mutations in yeast | Elsakrmy N, Aouida M, ..., Ramotar D | 10.1016/j.dnarep.2022.103359 |
| 37075541 | Saccharomyces cerevisiae apurinic/apyrimidinic endonuclease 1 repairs abasicsite-mediated DNA-peptide/protein cross-links | Bryan C, Le J, Wei X, Yang K | 10.1016/j.dnarep.2023.103501 |
| 33386486 | The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites isdependent on the major carbon source | Stokdyk K, Berroyer A, Grami ZA, Kim N | 10.1007/s00294-020-01141-4 |
| 33686425 | Identification and Characterization of Aminopeptidase N 1 Gene of the IndianMalaria Vector Anopheles culicifacies (Diptera: Culicidae) | Jakhar R, Gakhar SK | 10.1093/jme/tjab011 |
| 33711786 | Mitochondrial apurinic/apyrimidinic endonuclease Apn1 is not critical for thecompletion of the Plasmodium berghei life cycle | Srivastava PN, Narwal SK, Mishra S | 10.1016/j.dnarep.2021.103078 |
| 33096888 | New Insights in Saccharomyces cerevisiae Response to the CyanotoxinMicrocystin-LR, Revealed by Proteomics and Gene Expression | Valerio E, Barreiros S, ..., Campos A | 10.3390/toxins12100667 |