Genome location: Pf3D7_13_v3:1,344,509..1,347,450(-)
Genome classification: Core
Product Description: ATP-dependent RNA helicase DBP8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0006364 | rRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.91 |
| Trophozoite | expressed | 0.12 | 0.92 |
| Schizont | possibly expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.22 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1332700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.924 | MFS: -2.91 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IE01
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.01) with 4KBF (Global RMSD=2.36)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102828
Most Similar Human Ortholog: M0QXD4
TM-align score: 0.85 | RMSD: 1.16
Seq Identity: 0.30 | Length: 40 / 490
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105671 | DEAD-box helicase 49 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 26 | 21 | 29 |
| disruptive | 9 | 42 | 32 | 50 |
| missense | 9 | 42 | 32 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 58 | 24 | 36 |
| disruptive | 17 | 89 | 45 | 110 |
| missense | 15 | 81 | 41 | 90 |
PlasmoDB Total SNPs: 130
Non-coding: 100 | Synonymous: 17 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 490 | Molecular Weight (kDa): 57.131
PDB ID(s): None
Isoelectric Point: 10.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 20607362 | A rice DEAD-box protein, OsRH36, can complement an Arabidopsis atrh36 mutant, butcannot functionally replace its yeast homolog Dbp8p | Huang CK, Yu SM, Lu CA | 10.1007/s11103-010-9659-7 |
| 11222764 | Characterization and mutational analysis of yeast Dbp8p, a putative RNA helicaseinvolved in ribosome biogenesis | Daugeron MC, Linder P | 10.1093/nar/29.5.1144 |
| 10606264 | Crystallographic structure of the amino terminal domain of yeast initiationfactor 4A, a representative DEAD-box RNA helicase | Johnson ER, McKay DB | 10.1017/s1355838299991410 |