PF3D7_1332900 (cyto-IRS)

Genome location: Pf3D7_13_v3:1,349,542..1,354,540(-)

Genome classification: Core

Function and Localization

Product Description: isoleucine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.5 (Isoleucine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004822	isoleucine-tRNA ligase activity
Process	GO:0006428	isoleucyl-tRNA aminoacylation
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.73	0.67
Trophozoite	expressed	1.38	0.35
Schizont	expressed	0.21	0.87
Gametocyte	expressed	0.55	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1332900

Resistome Missense Mutations: E180D, E180Q, N269K, S288I, W395L, V500A, C502Y

Resistome Compounds with Missense Mutations: MMV1081413, MMV1091186, TCMDC-124553/MMV019869

Resistome # Samples with Disruptive Mutations: 18 (18 missense, 13 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.026 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDZ9

"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.18) with 8C9D (Global RMSD=3.07)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.5	isoleucine-tRNA ligase	tRNATrypsinsperminechlorideNSC70422muciproinmupirocinSB-205952SB-203207Ile-ol-AMPdiphosphateFuranomycin...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P41252	Isoleucine--tRNA ligase, cytoplasmic	Homo sapiens	OMA, OrthoDB	CHEMBL126515CHEMBL340359CHEMBL125820CHEMBL264002...
Q38A55	Isoleucine--tRNA ligase		OMA, OrthoDB, OrthoMCL	NSC70422

Orthology Information

Ortholog Group (OrthoMCL): OG6_100811

Most Similar Human Ortholog: A0A804HIG2

TM-align score: 0.96 | RMSD: 1.57

Seq Identity: 0.52 | Length: 435 / 1272

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000196305	isoleucyl-tRNA synthetase 1

Protein Information

Protein Length: 1272 | Molecular Weight (kDa): 151.311

UniProt ID(s): Q5KTV9, Q8IDZ9

PDB ID(s): None

Isoelectric Point: 7.58

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site
InterPro	IPR002300	Aminoacyl-tRNA synthetase, class Ia
InterPro	IPR002301	Isoleucine-tRNA ligase
InterPro	IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
InterPro	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
InterPro	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
InterPro	IPR033709	Isoleucyl tRNA synthetase type 2, anticodon-binding domain
PFam	PF00133	Aminoacyl-tRNA synthetase, class Ia
PFam	PF08264	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
Superfamily	SSF47323	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
Superfamily	SSF50677	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
Superfamily	SSF52374

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	76	25	66
disruptive	13	78	47	123
missense	9	67	43	109

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	119	45	75
disruptive	28	150	85	209
missense	17	133	69	152

PlasmoDB Total SNPs: 195

Non-coding: 111 | Synonymous: 66 | Nonsynonymous: 18 | Stop Codon: 0