Genome location: Pf3D7_13_v3:1,354,881..1,357,621(+)
Genome classification: Core
Product Description: 20 kDa chaperonin
SignalP Peptide: MKIIFLLNIVILFLTNVLT
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005634 | nucleus |
| Component | GO:0009536 | plastid |
| Function | GO:0051087 | chaperone binding |
| Function | GO:0046872 | metal ion binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0061077 | chaperone-mediated protein folding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.27 | 0.83 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1333000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.37 | MFS: -3.541 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1347800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDZ8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100490
Most Similar Human Ortholog: B8ZZL8
TM-align score: 0.77 | RMSD: 2.46
Seq Identity: 0.28 | Length: 94 / 258
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000115541 | heat shock protein family E (Hsp10) member 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 13 | 10 | 11 |
| disruptive | 1 | 15 | 6 | 20 |
| missense | 1 | 15 | 6 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 25 | 10 | 14 |
| disruptive | 10 | 25 | 17 | 48 |
| missense | 7 | 21 | 15 | 36 |
PlasmoDB Total SNPs: 202
Non-coding: 181 | Synonymous: 18 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 258 | Molecular Weight (kDa): 29.063
PDB ID(s): None
Isoelectric Point: 7.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011032 | GroES-like superfamily |
| InterPro | IPR018369 | Chaperonin GroES, conserved site |
| InterPro | IPR020818 | GroES chaperonin family |
| PFam | PF00166 | GroES chaperonin family |
| Superfamily | SSF50129 | GroES-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15926202 | Organelle-specific cochaperonins in apicomplexan parasites. | Sato S, Wilson RJ | 10.1016/j.molbiopara.2005.01.010 |
| 23326533 | P. falciparum cpn20 is a bona fide co-chaperonin that can replace GroES in E.coli. | Vitlin Gruber A, Nisemblat S, ..., Weiss C | 10.1371/journal.pone.0053909 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |