Genome location: Pf3D7_13_v3:1,428,350..1,439,462(-)
Genome classification: Core
Product Description: reticulocyte binding protein 2 homologue b
SignalP Peptide: MKTTLFCSISFCNIIFFFLELSHE
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020008 | rhoptry |
| Component | GO:1990225 | rhoptry neck |
| Function | GO:0008201 | heparin binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.29 | 0.89 |
| Trophozoite | possibly expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.46 | 0.80 |
| Gametocyte | not expressed | 0.03 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1335300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.972 | #Insertions: 11
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0316600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 10 | 4 | 13 |
| disruptive | 16 | 43 | 26 | 81 |
| missense | 14 | 40 | 22 | 74 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 16 | 5 | 31 |
| disruptive | 23 | 71 | 52 | 113 |
| missense | 21 | 58 | 47 | 94 |
PlasmoDB Total SNPs: 305
Non-coding: 86 | Synonymous: 115 | Nonsynonymous: 102 | Stop Codon: 2
Protein Length: 3254 | Molecular Weight (kDa): 382.883
UniProt ID(s): A0A159SIQ0, A0A159SIR0, A0A159SIU6, A0A159SJD8, A0A159SK46, A0A159SK52, A0A161F2F0, A0A161FJV0, A0A161FK53, A0A2S1Q7N4, Q7YWE6, Q7YWE7, Q7YWE8, Q7YWF0, Q7YWF1, Q7YWF2, Q9BK45
PDB ID(s): None
Isoelectric Point: 4.95
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12606570 | Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptortargeting for invasion of human erythrocytes. | Duraisingh MT, Triglia T, ..., Cowman AF | 10.1093/emboj/cdg096 |
| 15964948 | Dramatic difference in diversity between Plasmodium falciparum and Plasmodiumvivax reticulocyte binding-like genes. | Rayner JC, Tran TM, ..., Galinski MR | https://pubmed.ncbi.nlm.nih.gov/15964948/ |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17470537 | Molecular analysis of erythrocyte invasion in Plasmodium falciparum isolates fromSenegal. | Jennings CV, Ahouidi AD, ..., Duraisingh MT | 10.1128/IAI.00122-07 |
| 20487292 | Functional diversification between two related Plasmodium falciparum merozoiteinvasion ligands is determined by changes in the cytoplasmic domain. | Dvorin JD, Bei AK, Coleman BI, Duraisingh MT | 10.1111/j.1365-2958.2009.07040.x |
| 21386888 | Plasmodium falciparum reticulocyte binding-like homologue protein 2 (PfRH2) is akey adhesive molecule involved in erythrocyte invasion. | Sahar T, Reddy KS, ..., Gaur D | 10.1371/journal.pone.0017102 |
| 21698217 | Plasmodium falciparum merozoite invasion is inhibited by antibodies that targetthe PfRh2a and b binding domains. | Triglia T, Chen L, ..., Cowman AF | 10.1371/journal.ppat.1002075 |
| 23544851 | Specific phosphorylation of the PfRh2b invasion ligand of Plasmodium falciparum. | Engelberg K, Paul AS, ..., Gilberger TW | 10.1042/BJ20121694 |
| 24212193 | Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. | Kobayashi K, Takano R, ..., Kato K | 10.1038/srep03178 |
| 27438226 | PfRH2b specific monoclonal antibodies inhibit merozoite invasion. | Aniweh Y, Gao X, Gunalan K, Preiser PR | 10.1111/mmi.13468 |
| 28605544 | Functional Analysis Reveals Geographical Variation in Inhibitory Immune ResponsesAgainst a Polymorphic Malaria Antigen. | Bei AK, Ahouidi AD, ..., Duraisingh MT | 10.1093/infdis/jix280 |
| 30496294 | A forward genetic screen reveals a primary role for Plasmodium falciparumReticulocyte Binding Protein Homologue 2a and 2b in determining alternativeerythrocyte invasion pathways. | Campino S, Marin-Menendez A, ..., Rayner JC | 10.1371/journal.ppat.1007436 |
| 32001728 | Analysis of Plasmodium falciparum Rh2b deletion polymorphism across differenttransmission areas. | Aniweh Y, Suurbaar J, ..., Awandare GA | 10.1038/s41598-020-58300-3 |
| 26613787 | Population Structure Shapes Copy Number Variation in Malaria Parasites | Cheeseman IH, Miller B, ..., Anderson TJ | 10.1093/molbev/msv282 |
| 37653544 | Recent increase in low complexity polygenomic infections and sialicacid-independent invasion pathways in Plasmodium falciparum from Western Gambia | Nganyewo NN, Bojang F, ..., Amambua-Ngwa A | 10.1186/s13071-023-05929-4 |
| 31311552 | Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b(PfRh2b) in Senegal and The Gambia | Diedhiou CK, Moussa RA, ..., Ahouidi AD | 10.1186/s12936-019-2868-x |
| 29217416 | Identification of target proteins of clinical immunity to Plasmodium falciparumin a region of low malaria transmission | Sakamoto H, Takeo S, ..., Tsuboi T | 10.1016/j.parint.2017.12.002 |