Genome location: Pf3D7_13_v3:1,440,169..1,451,101(+)
Genome classification: Core
Product Description: reticulocyte binding protein 2 homologue a
SignalP Peptide: MKTTLFCSISFCNIIFFFLELSHE
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016020 | membrane |
| Component | GO:1990225 | rhoptry neck |
| Function | GO:0008201 | heparin binding |
| Function | GO:0046812 | host cell surface binding |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.36 | 0.87 |
| Trophozoite | not expressed | 0.04 | 0.97 |
| Schizont | expressed | 0.63 | 0.75 |
| Gametocyte | not expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1335400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.153 | #Insertions: 13
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0316600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0501000)
Modification: Disrupted | RMgm-1933
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O95630 | STAM-binding protein | OrthoMCL BLAST | CapziminCHEMBL4080694CHEMBL4088568CHEMBL4060337... | |
| Q16204 | Coiled-coil domain-containing protein 6 | OrthoMCL BLAST | CHEMBL3906151CHEMBL4473213CHEMBL4473213CHEMBL4640601... | |
| Q13586 | Stromal interaction molecule 1 | OrthoMCL BLAST | CHEMBL4177187CHEMBL4162723 | |
| P84903 | Calcium release-activated calcium channel protein/Stromal interaction molecule 1 | OrthoMCL BLAST | CHEMBL262766 |
Protein Length: 3130 | Molecular Weight (kDa): 370.436
UniProt ID(s): E5G970, Q25777, Q7JU29, Q7YWE8, Q7YWF0, Q7YWF1, Q7YWF2, Q8IDX6, Q95NX9, Q9BK45, Q9BK46, Q9U5A3
PDB ID(s): None
Isoelectric Point: 5.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 7 | 9 | 7 |
| disruptive | 11 | 30 | 17 | 52 |
| missense | 11 | 30 | 16 | 51 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 14 | 8 | 15 |
| disruptive | 15 | 54 | 23 | 68 |
| missense | 14 | 51 | 23 | 61 |
PlasmoDB Total SNPs: 281
Non-coding: 72 | Synonymous: 106 | Nonsynonymous: 103 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15964948 | Dramatic difference in diversity between Plasmodium falciparum and Plasmodiumvivax reticulocyte binding-like genes. | Rayner JC, Tran TM, ..., Galinski MR | https://pubmed.ncbi.nlm.nih.gov/15964948/ |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17470537 | Molecular analysis of erythrocyte invasion in Plasmodium falciparum isolates fromSenegal. | Jennings CV, Ahouidi AD, ..., Duraisingh MT | 10.1128/IAI.00122-07 |
| 20487292 | Functional diversification between two related Plasmodium falciparum merozoiteinvasion ligands is determined by changes in the cytoplasmic domain. | Dvorin JD, Bei AK, Coleman BI, Duraisingh MT | 10.1111/j.1365-2958.2009.07040.x |
| 21386888 | Plasmodium falciparum reticulocyte binding-like homologue protein 2 (PfRH2) is akey adhesive molecule involved in erythrocyte invasion. | Sahar T, Reddy KS, ..., Gaur D | 10.1371/journal.pone.0017102 |
| 21698217 | Plasmodium falciparum merozoite invasion is inhibited by antibodies that targetthe PfRh2a and b binding domains. | Triglia T, Chen L, ..., Cowman AF | 10.1371/journal.ppat.1002075 |
| 24212193 | Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. | Kobayashi K, Takano R, ..., Kato K | 10.1038/srep03178 |
| 30496294 | A forward genetic screen reveals a primary role for Plasmodium falciparumReticulocyte Binding Protein Homologue 2a and 2b in determining alternativeerythrocyte invasion pathways. | Campino S, Marin-Menendez A, ..., Rayner JC | 10.1371/journal.ppat.1007436 |