PF3D7_1335500

Genome location: Pf3D7_13_v3:1,451,489..1,453,443(+)

Genome classification: Core

Function and Localization

Product Description: DNA replication complex GINS protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000811	GINS complex
Component	GO:0031298	replication fork protection complex
Process	GO:0000727	double-strand break repair via break-induced replication

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.01	1.00
Trophozoite	not expressed	0.00	1.00
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.24	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1335500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.998 | MFS: -2.582 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1349400)

Relative Growth Rate: 1.01 ± 0.13
Confidence: 5.41

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1349400)

Modification: Disrupted | RMgm-3639

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5F5

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_145254

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	6	7	15
disruptive	1	16	6	26
missense	1	16	6	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	20	10	23
disruptive	3	26	25	67
missense	3	20	20	46

PlasmoDB Total SNPs: 74

Non-coding: 53 | Synonymous: 12 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 493 | Molecular Weight (kDa): 58.741

UniProt ID(s): C0H5F5

PDB ID(s): None

Isoelectric Point: 4.71

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007257	DNA replication complex GINS protein Psf2
InterPro	IPR021151	GINS subunit, domain A
InterPro	IPR036224	GINS, helical bundle-like domain superfamily
PFam	PF05916	GINS subunit, domain A
Superfamily	SSF158573	GINS, helical bundle-like domain superfamily