Genome location: Pf3D7_13_v3:1,464,895..1,466,619(-)
Genome classification: Core
Product Description: thrombospondin-related anonymous protein
SignalP Peptide: MNHLGNVKYLVIVFLIFFDLFLVNG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0016020 | membrane |
| Component | GO:0020009 | microneme |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0071976 | cell gliding |
| Process | GO:0044409 | entry into host |
| Process | GO:0006928 | movement of cell or subcellular component |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1335900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.756 | MFS: -2.134 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1349800)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1349800)
Modification: Mutated | RMgm-5238
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q76NM2
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P58335 | Anthrax toxin receptor 2 | OrthoMCL BLAST | CHEMBL2070828CHEBI:1369acs.jmedchem.1c00409_ST.650CHEMBL4557794... |
Protein Length: 574 | Molecular Weight (kDa): 64.742
UniProt ID(s): A0A5Q2EXK8, A0A5Q2EZD7, A0A5Q2F1F6, A0A5Q2F2B8, A0A5Q2F2H6, A0A5Q2F4G9, O76110, P16893, Q01507, Q26020, Q76NM2, W8VNB6
Isoelectric Point: 4.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000884 | Thrombospondin type-1 (TSP1) repeat |
| InterPro | IPR002035 | von Willebrand factor, type A |
| InterPro | IPR036383 | Thrombospondin type-1 (TSP1) repeat superfamily |
| InterPro | IPR036465 | von Willebrand factor A-like domain superfamily |
| PFam | PF00090 | Thrombospondin type-1 (TSP1) repeat |
| PFam | PF00092 | von Willebrand factor, type A |
| Superfamily | SSF53300 | von Willebrand factor A-like domain superfamily |
| Superfamily | SSF82895 | Thrombospondin type-1 (TSP1) repeat superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 17 | 10 | 23 |
| disruptive | 155 | 65 | 19 | 53 |
| missense | 119 | 45 | 12 | 34 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 24 | 14 | 34 |
| disruptive | 178 | 74 | 39 | 87 |
| missense | 135 | 50 | 24 | 50 |
PlasmoDB Total SNPs: 139
Non-coding: 0 | Synonymous: 12 | Nonsynonymous: 124 | Stop Codon: 3
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 1409621 | Characterization of Plasmodium falciparum sporozoite surface protein 2. | Rogers WO, Malik A, ..., Hoffman SL | 10.1073/pnas.89.19.9176 |
| 7664729 | Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum:expression during sporozoite ontogeny and binding to human hepatocytes. | Robson KJ, Frevert U, ..., et al. | 10.1002/j.1460-2075.1995.tb00060.x |
| 9267031 | TRAP is necessary for gliding motility and infectivity of plasmodium sporozoites. | Sultan AA, Thathy V, ..., Menard R | 10.1016/s0092-8674(00)80511-5 |
| 17426153 | Aldolase provides an unusual binding site for thrombospondin-related anonymousprotein in the invasion machinery of the malaria parasite. | Bosch J, Buscaglia CA, ..., Hol WG | 10.1073/pnas.0605301104 |
| 19148273 | Malaria parasite invasion of the mosquito salivary gland requires interactionbetween the Plasmodium TRAP and the Anopheles saglin proteins. | Ghosh AK, Devenport M, ..., Jacobs-Lorena M | 10.1371/journal.ppat.1000265 |
| 24587387 | Diversifying selection on the thrombospondin-related adhesive protein (TRAP) geneof Plasmodium falciparum in Thailand. | Ohashi J, Suzuki Y, ..., Patarapotikul J | 10.1371/journal.pone.0090522 |
| 26289816 | Inhibition by stabilization: targeting the Plasmodium falciparum aldolase-TRAPcomplex. | Nemetski SM, Cardozo TJ, ..., Bosch J | 10.1186/s12936-015-0834-9 |
| 27007512 | Malaria sporozoite protein expression enhances baculovirus-mediated gene transferto hepatocytes. | Tamura T, Kawabata C, ..., Yoshida S | 10.1002/jgm.2879 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 29632205 | Alpha-v-containing integrins are host receptors for the Plasmodium falciparumsporozoite surface protein, TRAP. | Dundas K, Shears MJ, ..., Wright GJ | 10.1073/pnas.1719660115 |
| 31954917 | Population genetic structure analysis of thrombospondin-related adhesive protein(TRAP) as a vaccine candidate antigen in worldwide Plasmodium falciparumisolates. | Mehrizi AA, Jafari Zadeh A, Zakeri S, Djadid ND | 10.1016/j.meegid.2020.104197 |
| 32648541 | Evolutionarily distant I domains can functionally replace the essentialligand-binding domain of Plasmodium T RAP. L ID - 10 | Klug D, Goellner S, ..., Frischknecht F | 10.7554/eLife.57572 |
| 34059712 | Platelet derived growth factor receptor beta (PDGFRbeta) is a host receptor for thehuman malaria parasite adhesin TRAP. | Steel RWJ, Vigdorovich V, ..., Kappe SHI | 10.1038/s41598-021-90722-5 |
| 38432655 | [Porphyromonas gingivalis outer membrane vesicles activate Toll-like receptor 2to promote osteoclast differentiation by carrying lipopolysaccharide] | Zou JK, Cao YM, ..., He XT | 10.3760/cma.j.cn112144-20231026-00222 |