Genome location: Pf3D7_13_v3:1,487,930..1,490,567(-)
Genome classification: Core
Product Description: tryptophan--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.2 (Tryptophan--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0004830 | tryptophan-tRNA ligase activity |
| Process | GO:0006436 | tryptophanyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.81 |
| Trophozoite | expressed | 0.74 | 0.60 |
| Schizont | expressed | 0.10 | 0.94 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1336900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.142 | MFS: -3.188 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1350700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDW3
"Best" AlphaFill ligand hit: TYM (tryptophanyl-5'amp, Local RMSD=5.63) with 4J75 (Global RMSD=2.24)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.2 | tryptophan-tRNA ligase | NEMdATPadenineadenosinetryptamineindolmycinL-tryptophanD-tryptophanchuangxinmycinsuccinylacetone3'-O-methyl-ATP5-Methyltryptophan... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101168
Most Similar Human Ortholog: P23381
TM-align score: 0.80 | RMSD: 1.25
Seq Identity: 0.52 | Length: 385 / 632
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000140105 | tryptophanyl-tRNA synthetase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 27 | 10 | 39 |
| disruptive | 3 | 35 | 23 | 51 |
| missense | 3 | 35 | 23 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 38 | 24 | 51 |
| disruptive | 13 | 75 | 34 | 100 |
| missense | 10 | 60 | 29 | 59 |
PlasmoDB Total SNPs: 104
Non-coding: 68 | Synonymous: 25 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 632 | Molecular Weight (kDa): 73.1
UniProt ID(s): Q8IDW3
Isoelectric Point: 8.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002305 | Aminoacyl-tRNA synthetase, class Ic |
| InterPro | IPR002306 | Tryptophan-tRNA ligase |
| InterPro | IPR018163 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily |
| PFam | PF00579 | Aminoacyl-tRNA synthetase, class Ic |
| Superfamily | SSF52374 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily |
| Superfamily | SSF55186 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 27277538 | Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayeddeath in Plasmodium falciparum. | Pasaje CF, Cheung V, ..., Ralph SA | 10.1038/srep27531 |
| 23665145 | Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNAsynthetase and its potential as a target for structure-guided drug design | Koh CY, Kim JE, ..., Hol WG | 10.1016/j.molbiopara.2013.04.007 |