PF3D7_1337300

Genome location: Pf3D7_13_v3:1,502,688..1,506,534(+)

Genome classification: Core

Function and Localization

Product Description: exoribonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.11.1 (Exodeoxyribonuclease I);3.1.16.1 (Spleen exonuclease)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.31	0.85
Trophozoite	expressed	0.48	0.72
Schizont	expressed	0.09	0.94
Gametocyte	expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1337300

Resistome Missense Mutations: L354P

Resistome Compounds with Missense Mutations: ML901

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.956 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1351100)

Relative Growth Rate: 0.14 ± 0.10
Confidence: 6.02

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8K9

"Best" AlphaFill ligand hit: U5P (uridine-5'-monophosphate, Local RMSD=0.44) with 1WLJ (Global RMSD=2.43)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.11.1	exodeoxyribonuclease I	NTPEDTAcamptothecinendonuclease CtIPfullerene-oligonucleotide conjugatesingle-stranded DNA-binding protein5'-thiophosphorylated oligonucleotide
3.1.16.1	spleen exonuclease	ATPKClEDTARNAiheparinarseniteATPgammaSUDP-glucosePentamidineacetylationSP-ATPalphaSSP-CTPalphaS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103246

Most Similar Human Ortholog: A0A2R8Y7I5

TM-align score: 0.38 | RMSD: 3.20

Seq Identity: 0.35 | Length: 221 / 903

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000079313	RNA exonuclease 1 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	60	20	54
disruptive	32	96	35	109
missense	21	79	31	96

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	89	31	61
disruptive	48	158	105	187
missense	31	127	96	152

PlasmoDB Total SNPs: 207

Non-coding: 135 | Synonymous: 41 | Nonsynonymous: 30 | Stop Codon: 1

Protein Information

Protein Length: 903 | Molecular Weight (kDa): 106.776

UniProt ID(s): A0A5K1K8K9

PDB ID(s): None

Isoelectric Point: 7.07

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR012337	Ribonuclease H-like superfamily
InterPro	IPR013520	Exonuclease, RNase T/DNA polymerase III
InterPro	IPR034922	RNA exonuclease 1-like, exonuclease domain
PFam	N/A	N/A
Superfamily	SSF53098	Ribonuclease H-like superfamily