Genome location: Pf3D7_13_v3:1,540,544..1,544,422(+)
Genome classification: Core
Product Description: 26S proteasome regulatory subunit RPN3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.18 | 0.91 |
| Trophozoite | expressed | 0.62 | 0.64 |
| Schizont | expressed | 0.46 | 0.71 |
| Gametocyte | expressed | 0.37 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1338100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.189 | MFS: -2.887 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1351800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K9F3
"Best" AlphaFill ligand hit: R78 (4-{[(7r)-8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl]amino}-3-methoxy-n-(1-methylpiperidin-4-yl)benzamide, Local RMSD=6.55) with 4PY6 (Global RMSD=13.95)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101680
Most Similar Human Ortholog: O43242
TM-align score: 0.85 | RMSD: 2.60
Seq Identity: 0.38 | Length: 455 / 503
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108344 | proteasome 26S subunit, non-ATPase 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 17 | 44 |
| disruptive | 5 | 15 | 15 | 54 |
| missense | 5 | 15 | 14 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 68 | 29 | 33 |
| disruptive | 7 | 75 | 49 | 132 |
| missense | 6 | 60 | 37 | 80 |
PlasmoDB Total SNPs: 196
Non-coding: 172 | Synonymous: 19 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 503 | Molecular Weight (kDa): 59.066
UniProt ID(s): A0A5K1K9F3
PDB ID(s): None
Isoelectric Point: 8.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000717 | Proteasome component (PCI) domain |
| InterPro | IPR013586 | 26S proteasome regulatory subunit, C-terminal |
| InterPro | IPR035267 | 26S proteasome non-ATPase regulatory subunit 3 |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF01399 | Proteasome component (PCI) domain |
| PFam | PF08375 | 26S proteasome regulatory subunit, C-terminal |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 20471955 | Dissection of the assembly pathway of the proteasome lid in Saccharomycescerevisiae | Fukunaga K, Kudo T, ..., Saeki Y | 10.1016/j.bbrc.2010.05.061 |
| 23934994 | The C-terminal residues of Saccharomyces cerevisiae Mec1 are required for itslocalization, stability, and function | DaSilva LF, Pillon S, ..., Brandl CJ | 10.1534/g3.113.006841 |