Genome location: Pf3D7_13_v3:1,547,546..1,550,917(+)
Genome classification: Core
Product Description: elongation factor 1-gamma, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005853 | eukaryotic translation elongation factor 1 complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0004364 | glutathione transferase activity |
| Function | GO:0003746 | translation elongation factor activity |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.69 | 0.36 |
| Trophozoite | expressed | 2.26 | 0.15 |
| Schizont | expressed | 0.51 | 0.72 |
| Gametocyte | expressed | 1.05 | 0.48 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1338300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.763 | MFS: -2.447 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1352000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K967
"Best" AlphaFill ligand hit: GSF (glutathione sulfinate, Local RMSD=0.08) with 4PNG (Global RMSD=4.35)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101050
Most Similar Human Ortholog: P26641
TM-align score: 0.49 | RMSD: 6.56
Seq Identity: 0.19 | Length: 314 / 411
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000254772 | eukaryotic translation elongation factor 1 gamma |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 18 | 15 | 35 |
| disruptive | 12 | 45 | 18 | 70 |
| missense | 8 | 41 | 16 | 64 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 46 | 18 | 32 |
| disruptive | 26 | 93 | 45 | 104 |
| missense | 17 | 75 | 37 | 80 |
PlasmoDB Total SNPs: 161
Non-coding: 127 | Synonymous: 16 | Nonsynonymous: 18 | Stop Codon: 0
Protein Length: 411 | Molecular Weight (kDa): 47.777
UniProt ID(s): A0A5K1K967
PDB ID(s): None
Isoelectric Point: 7.35
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001662 | Elongation factor 1B gamma, C-terminal |
| InterPro | IPR004045 | Glutathione S-transferase, N-terminal |
| InterPro | IPR004046 | Glutathione S-transferase, C-terminal |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| InterPro | IPR036282 | Glutathione S-transferase, C-terminal domain superfamily |
| InterPro | IPR036433 | Elongation factor EF1B gamma, C-terminal domain superfamily |
| InterPro | IPR040079 | Glutathione Transferase family |
| PFam | PF00043 | Glutathione S-transferase, C-terminal |
| PFam | PF00647 | Elongation factor 1B gamma, C-terminal |
| PFam | PF02798 | Glutathione S-transferase, N-terminal |
| Superfamily | SSF47616 | Glutathione S-transferase, C-terminal domain superfamily |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
| Superfamily | SSF89942 | Elongation factor EF1B gamma, C-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 17307963 | Purification of components of the translation elongation factor complex ofPlasmodium falciparum by tandem affinity purification. | Takebe S, Witola WH, ..., Ben Mamoun C | 10.1128/EC.00376-06 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 38360885 | Chp1 is a dedicated chaperone at the ribosome that safeguards eEF1A biogenesis | Minoia M, Quintana-Cordero J, ..., Andreasson C | 10.1038/s41467-024-45645-w |
| 38199565 | Methylation of elongation factor 1A by yeast Efm4 or human eEF1A-KMT2 involves abeta-hairpin recognition motif and crosstalks with phosphorylation | Hamey JJ, Nguyen A, ..., Wilkins MR | 10.1016/j.jbc.2024.105639 |
| 38359077 | Ogataea nonmethanolica f.a, sp. nov., a novel yeast species isolated from rottingwood in Brazil and Colombia | Garcia-Acero AM, Morais CG, ..., Rosa CA | 10.1099/ijsem.0.006273 |
| 37861337 | Eukaryotic translation elongation factor 1 alpha (eEF1A) inhibits Sinipercachuatsi rhabdovirus (SCRV) infection through two distinct mechanisms | Meng X-Y, Jiang Q-Q, ..., Ke F | 10.1128/jvi.01226-23 |