PF3D7_1338900

Genome location: Pf3D7_13_v3:1,568,367..1,571,577(-)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	expressed	0.67	0.63
Schizont	expressed	0.40	0.76
Gametocyte	expressed	1.08	0.47

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1338900

Resistome Missense Mutations: N355K, N358K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.276 | MFS: -2.61 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1352600)

Relative Growth Rate: 1.00 ± 0.04
Confidence: 7.70

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1352600)

Modification: Disrupted | RMgm-3658

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8V9

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.14) with 3GC0 (Global RMSD=6.05)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_114875

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	39	32	67
disruptive	28	140	81	160
missense	23	127	81	144

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	85	41	74
disruptive	73	278	113	232
missense	45	239	104	183

PlasmoDB Total SNPs: 145

Non-coding: 69 | Synonymous: 37 | Nonsynonymous: 39 | Stop Codon: 0

Protein Information

Protein Length: 931 | Molecular Weight (kDa): 111.339

UniProt ID(s): A0A5K1K8V9

PDB ID(s): None

Isoelectric Point: 9.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR017441	Protein kinase, ATP binding site
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily