PF3D7_1340100 (RRP42)

Genome location: Pf3D7_13_v3:1,603,632..1,605,515(-)

Genome classification: Core

Function and Localization

Product Description: exosome complex component RRP42, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.56 (tRNA nucleotidyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000178	exosome (RNase complex)
Process	GO:0016075	rRNA catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.09	0.95
Trophozoite	expressed	0.13	0.91
Schizont	expressed	0.03	0.98
Gametocyte	expressed	0.22	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1340100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.274 | MFS: -2.601 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1353500)

Relative Growth Rate: 0.24 ± 0.39
Confidence: 3.28

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8M9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.56	tRNA nucleotidyltransferase	KClEDTAphosphateN-ethylmaleimidep-hydroxymercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103422

Most Similar Human Ortholog: Q15024

TM-align score: 0.90 | RMSD: 1.76

Seq Identity: 0.25 | Length: 267 / 278

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000075914	exosome component 7

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	15	8	30
disruptive	3	12	12	33
missense	3	12	12	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	33	9	26
disruptive	7	37	33	65
missense	6	32	31	51

PlasmoDB Total SNPs: 109

Non-coding: 93 | Synonymous: 12 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 278 | Molecular Weight (kDa): 31.245

UniProt ID(s): A0A5K1K8M9

PDB ID(s): None

Isoelectric Point: 5.02

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001247	Exoribonuclease, phosphorolytic domain 1
InterPro	IPR015847	Exoribonuclease, phosphorolytic domain 2
InterPro	IPR020568	Ribosomal protein S5 domain 2-type fold
InterPro	IPR036345	Exoribonuclease, PH domain 2 superfamily
PFam	PF01138	Exoribonuclease, phosphorolytic domain 1
PFam	PF03725	Exoribonuclease, phosphorolytic domain 2
Superfamily	SSF54211	Ribosomal protein S5 domain 2-type fold
Superfamily	SSF55666	Exoribonuclease, PH domain 2 superfamily