Genome location: Pf3D7_13_v3:1,617,537..1,620,495(-)
Genome classification: Core
Product Description: RNA lariat debranching enzyme, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.4.- (Phosphoric diester hydrolases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.37 | 0.82 |
| Trophozoite | expressed | 0.56 | 0.68 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | possibly expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1340600
Resistome Missense Mutations: D485N
Resistome Compounds with Missense Mutations: BI-2536
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.25 | MFS: -2.756 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1354000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K8J0
"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.50) with 4PEG (Global RMSD=10.45)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.4.- | Ex: 3',5'-cyclic-nucleotide phosphodiesterase | KFATPADPCoAFADUDPAMPGTPCTPUTPCMPUMP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102255
Most Similar Human Ortholog: F8WAY1
TM-align score: 0.94 | RMSD: 1.21
Seq Identity: 0.53 | Length: 118 / 575
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000138231 | debranching RNA lariats 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 28 | 19 | 47 |
| disruptive | 16 | 111 | 57 | 108 |
| missense | 14 | 102 | 57 | 89 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 69 | 23 | 40 |
| disruptive | 44 | 189 | 82 | 144 |
| missense | 34 | 169 | 64 | 95 |
PlasmoDB Total SNPs: 208
Non-coding: 153 | Synonymous: 21 | Nonsynonymous: 33 | Stop Codon: 1
Protein Length: 575 | Molecular Weight (kDa): 67.555
UniProt ID(s): A0A5K1K8J0
PDB ID(s): None
Isoelectric Point: 5.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007708 | Lariat debranching enzyme, C-terminal |
| InterPro | IPR041816 | Lariat debranching enzyme, N-terminal metallophosphatase domain |
| PFam | PF05011 | Lariat debranching enzyme, C-terminal |
| Superfamily | SSF56300 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 37398028 | The debranching enzyme Dbr1 regulates lariat turnover and intron splicing | Buerer L, Clark NE, ..., Fairbrother WG | 10.21203/rs.3.rs-2931976/v1 |
| 33464395 | Saccharomyces cerevisiae RNA lariat debranching enzyme, Dbr1p, is required forcompletion of reverse transcription by the retrovirus-like element Ty1 andcleaves branched Ty1 RNAs | Menees TM | 10.1007/s00438-020-01753-y |
| 37369199 | A functional link between lariat debranching enzyme and the intron-bindingcomplex is defective in non-photosensitive trichothiodystrophy | Townley BA, Buerer L, ..., Mosammaparast N | 10.1016/j.molcel.2023.06.011 |
| 36289323 | Intron turnover is essential to the development and pathogenicity of the plantpathogenic fungus Fusarium graminearum | Choi Y, Lee HH, ..., Son H | 10.1038/s42003-022-04111-3 |
| 36974901 | Absence of SICKLE triggers programed cell death by disturbing alternativesplicing and decay of mRNAs | Wu C, Zhen W, ..., Hu Z | 10.1093/plphys/kiad192 |
| 35459748 | Metal content and kinetic properties of yeast RNA lariat debranching enzyme Dbr1 | Clark NE, Katolik A, ..., Fairbrother WG | 10.1261/rna.079159.122 |
| 37507019 | Activation of human RNA lariat debranching enzyme Dbr1 by binding protein TTDN1occurs though an intrinsically disordered C-terminal domain | Clark NE, Katolik A, ..., Fairbrother WG | 10.1016/j.jbc.2023.105100 |
| 34195958 | Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNADeep Sequencing | Wang T, Zhang X, Zheng B | 10.1007/978-1-0716-1645-1_5 |
| 36484984 | Crystal Structure of the RNA Lariat Debranching Enzyme Dbr1 with Hydrolyzed Phosphorothioate RNA Product | Clark NE, Katolik A, ..., Fairbrother WG | 10.1021/acs.biochem.2c00590 |
| 35244467 | The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous CellCarcinoma | Xu X, Yang X, ..., Xi Y | 10.1177/15330338221083105 |
| 33386334 | Rare Genetic Variants in Immune Genes and Neonatal Herpes Simplex Viral Infections | Cummings L, Tucker M, ..., Sampath V | 10.1542/peds.2020-0687 |
| 35788403 | SICKLE modulates lateral root development by promoting degradation of lariatintronic RNA | Wu C, Wang X, ..., Hu Z | 10.1093/plphys/kiac301 |