Genome location: Pf3D7_13_v3:1,641,926..1,644,490(+)
Genome classification: Core
Product Description: GPI mannosyltransferase 3
SignalP Peptide: N/A
# Transmembrane Domains: 11
EC Numbers: 2.4.1.- (Hexosyltransferases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005789 | endoplasmic reticulum membrane |
| Function | GO:0000026 | alpha-1,2-mannosyltransferase activity |
| Function | GO:0000030 | mannosyltransferase activity |
| Process | GO:0006506 | GPI anchor biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.27 | 0.82 |
| Schizont | expressed | 0.10 | 0.93 |
| Gametocyte | possibly expressed | 0.13 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1341600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.488 | MFS: -2.397 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1354700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDS3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.- | Ex: levansucrase | KIATPADPUDPAMPGTPGDPCTPUTPKClNEMIMP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103087
Most Similar Human Ortholog: Q92521
TM-align score: 0.82 | RMSD: 2.42
Seq Identity: 0.26 | Length: 485 / 786
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000069943 | phosphatidylinositol glycan anchor biosynthesis class B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 59 | 21 | 50 |
| disruptive | 21 | 69 | 58 | 128 |
| missense | 14 | 64 | 56 | 116 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 93 | 35 | 60 |
| disruptive | 40 | 188 | 109 | 197 |
| missense | 26 | 159 | 94 | 139 |
PlasmoDB Total SNPs: 107
Non-coding: 33 | Synonymous: 54 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 786 | Molecular Weight (kDa): 94.759
PDB ID(s): None
Isoelectric Point: 8.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005599 | GPI mannosyltransferase |
| InterPro | IPR039521 | GPI mannosyltransferase 3 |
| PFam | PF03901 | GPI mannosyltransferase |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12117963 | Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodiumfalciparum. | Delorenzi M, Sexton A, ..., Schofield L | 10.1128/IAI.70.8.4510-4522.2002 |
| 17977514 | Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequenceand is functional. | Basagoudanavar SH, Feng X, ..., Gowda DC | 10.1016/j.bbrc.2007.10.061 |
| 24489949 | Complementation of essential yeast GPI mannosyltransferase mutations suggests anovel specificity for certain Trypanosoma and Plasmodium PigB proteins. | Cortes LK, Scarcelli JJ, Taron CH | 10.1371/journal.pone.0087673 |
| 25625920 | VCP/Cdc48 rescues the growth defect of a GPI10 mutant in yeast | Ohnuma Y, Takata T, ..., Kakizuka A | 10.1016/j.febslet.2015.01.017 |
| 11159918 | Phosphatidylethanolamine is the donor of the phosphorylethanolamine linked to thealpha1,4-linked mannose of yeast GPI structures | Imhof I, Canivenc-Gansel E, Meyer U, Conzelmann A | 10.1093/glycob/10.12.1271 |
| 24963069 | ABNORMAL POLLEN TUBE GUIDANCE1, an Endoplasmic Reticulum-LocalizedMannosyltransferase Homolog of GLYCOSYLPHOSPHATIDYLINOSITOL10 in Yeast andPHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS B in Human, Is Required forArabidopsis Pollen Tube Micropylar Guidance and Embryo Development | Dai XR, Gao XQ, ..., Zhang XS | 10.1104/pp.114.236133 |
| 17311586 | In vivo characterization of the GPI assembly defect in yeast mcd4-174 mutants andbypass of the Mcd4p-dependent step in mcd4Delta cells | Wiedman JM, Fabre AL, ..., Orlean P | 10.1111/j.1567-1364.2006.00139.x |
| 9639537 | GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to thealpha1,4-linked mannose of the complete precursor glycophospholipid | Canivenc-Gansel E, Imhof I, ..., Benachour A | 10.1093/glycob/8.8.761 |
| 10954751 | Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei | Nagamune K, Nozaki T, ..., Kinoshita T | 10.1073/pnas.180230697 |
| 11356840 | The essential Smp3 protein is required for addition of the side-branching fourthmannose during assembly of yeast glycosylphosphatidylinositols | Grimme SJ, Westfall BA, ..., Orlean P | 10.1074/jbc.M101986200 |
| 9576863 | Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, isrequired for glycosylphosphatidylinositol-anchor synthesis | Sutterlin C, Escribano MV, ..., Riezman H | 10.1042/bj3320153 |