Genome location: Pf3D7_13_v3:1,655,461..1,660,193(+)
Genome classification: Core
Product Description: aconitate hydratase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.2.1.3 (Aconitate hydratase);4.2.1.33 (3-isopropylmalate dehydratase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051539 | 4 iron, 4 sulfur cluster binding |
| Function | GO:0003994 | aconitate hydratase activity |
| Function | GO:0005506 | iron ion binding |
| Process | GO:0006101 | citrate metabolic process |
| Process | GO:0006447 | regulation of translational initiation by iron |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.12 | 0.94 |
| Trophozoite | not expressed | 0.34 | 0.79 |
| Schizont | expressed | 0.73 | 0.58 |
| Gametocyte | possibly expressed | 0.30 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1342100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.807 | MFS: -2.209 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1355200)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1355200)
Modification: Disrupted | RMgm-4081
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDR8
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101028
Most Similar Human Ortholog: P21399
TM-align score: 0.99 | RMSD: 0.84
Seq Identity: 0.54 | Length: 882 / 909
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000122729 | aconitase 1 |
| ENSG00000136381 | iron responsive element binding protein 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 57 | 29 | 60 |
| disruptive | 12 | 50 | 35 | 104 |
| missense | 12 | 50 | 34 | 87 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 110 | 48 | 71 |
| disruptive | 22 | 128 | 54 | 172 |
| missense | 21 | 113 | 49 | 121 |
PlasmoDB Total SNPs: 194
Non-coding: 158 | Synonymous: 22 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 909 | Molecular Weight (kDa): 103.379
PDB ID(s): None
Isoelectric Point: 7.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000573 | Aconitase A/isopropylmalate dehydratase small subunit, swivel domain |
| InterPro | IPR001030 | Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain |
| InterPro | IPR006249 | Aconitase/Iron-responsive element-binding protein 2 |
| InterPro | IPR036008 | Aconitase, iron-sulfur domain |
| PFam | PF00330 | Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain |
| PFam | PF00694 | Aconitase A/isopropylmalate dehydratase small subunit, swivel domain |
| Superfamily | SSF52016 | Aconitase, iron-sulfur domain |
| Superfamily | SSF53732 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15963579 | An iron regulatory-like protein expressed in Plasmodium falciparum displaysaconitase activity. | Hodges M, Yikilmaz E, ..., Loyevsky M | 10.1016/j.molbiopara.2005.05.004 |
| 25843709 | Genetic investigation of tricarboxylic acid metabolism during the Plasmodiumfalciparum life cycle. | Ke H, Lewis IA, ..., Vaidya AB | 10.1016/j.celrep.2015.03.011 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |