Genome location: Pf3D7_13_v3:1,669,390..1,671,510(-)
Genome classification: Core
Product Description: sporozoite protein essential for cell traversal
SignalP Peptide: MKMKIPICFLIILVLLKCVLS
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0020009 | microneme |
| Process | GO:0048870 | cell motility |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.16 | 0.93 |
| Trophozoite | not expressed | 0.07 | 0.96 |
| Schizont | not expressed | 0.05 | 0.97 |
| Gametocyte | not expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1342500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.151 | MFS: -3.061 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1355600)
Modification: Disrupted | RMgm-139
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDR4
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 12 | 7 | 18 |
| disruptive | 21 | 26 | 12 | 35 |
| missense | 19 | 22 | 12 | 34 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 23 | 15 | 22 |
| disruptive | 26 | 57 | 36 | 77 |
| missense | 22 | 45 | 36 | 56 |
PlasmoDB Total SNPs: 120
Non-coding: 97 | Synonymous: 6 | Nonsynonymous: 17 | Stop Codon: 0
Protein Length: 245 | Molecular Weight (kDa): 28.474
UniProt ID(s): Q8IDR4
PDB ID(s): None
Isoelectric Point: 4.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR040897 | Plasmodium host cell traversal SPECT1 |
| PFam | PF18680 | Plasmodium host cell traversal SPECT1 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 14737184 | Cell-passage activity is required for the malarial parasite to cross the liversinusoidal cell layer. | Ishino T, Yano K, Chinzei Y, Yuda M | 10.1371/journal.pbio.0020004 |
| 28355563 | Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection inHumanized Mice. | Yang ASP, O'Neill MT, ..., Boddey JA | 10.1016/j.celrep.2017.03.017 |
| 25479287 | Structure of the essential Plasmodium host cell traversal protein SPECT1 | Hamaoka BY, Ghosh P | 10.1371/journal.pone.0114685 |
| 38218737 | Naturally acquired antibodies against Plasmodium vivax pre-erythrocytic stagevaccine antigens inhibit sporozoite invasion of human hepatocytes in vitro | Ntumngia FB, Kolli SK, ..., Adams JH | 10.1038/s41598-024-51820-2 |
| 17041140 | Developmental biology of sporozoite-host interactions in Plasmodium falciparummalaria: implications for vaccine design | Garcia JE, Puentes A, Patarroyo ME | 10.1128/CMR.00063-05 |
| 37703302 | Preliminary characterization of Plasmodium vivax sporozoite antigens aspre-erythrocytic vaccine candidates | Nicholas J, De SL, ..., Adams JH | 10.1371/journal.pntd.0011598 |
| 27374339 | The structure of sperm Izumo1 reveals unexpected similarities with Plasmodiuminvasion proteins | Nishimura K, Han L, ..., Jovine L | 10.1016/j.cub.2016.06.028 |