About Gene List

PF3D7_1342500 (SPECT1)

Genome location: Pf3D7_13_v3:1,669,390..1,671,510(-)

Genome classification: Core

Function and Localization

Product Description: sporozoite protein essential for cell traversal

SignalP Peptide: MKMKIPICFLIILVLLKCVLS

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Component GO:0020009 microneme
Process GO:0048870 cell motility

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring not expressed 0.16 0.93
Trophozoite not expressed 0.07 0.96
Schizont not expressed 0.05 0.97
Gametocyte not expressed 0.07 0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1342500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.151 | MFS: -3.061 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1355600)

Modification: Disrupted | RMgm-139

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDR4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532754

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 12 7 18
disruptive 21 26 12 35
missense 19 22 12 34

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 6 23 15 22
disruptive 26 57 36 77
missense 22 45 36 56

PlasmoDB Total SNPs: 120

Non-coding: 97 | Synonymous: 6 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 245 | Molecular Weight (kDa): 28.474

UniProt ID(s): Q8IDR4

PDB ID(s): None

Isoelectric Point: 4.81

Protein Domain Annotations:

Source Family ID Description
InterPro IPR040897 Plasmodium host cell traversal SPECT1
PFam PF18680 Plasmodium host cell traversal SPECT1
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
14737184 Cell-passage activity is required for the malarial parasite to cross the liversinusoidal cell layer. Ishino T, Yano K, Chinzei Y, Yuda M 10.1371/journal.pbio.0020004
28355563 Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection inHumanized Mice. Yang ASP, O'Neill MT, ..., Boddey JA 10.1016/j.celrep.2017.03.017
25479287 Structure of the essential Plasmodium host cell traversal protein SPECT1 Hamaoka BY, Ghosh P 10.1371/journal.pone.0114685
38218737 Naturally acquired antibodies against Plasmodium vivax pre-erythrocytic stagevaccine antigens inhibit sporozoite invasion of human hepatocytes in vitro Ntumngia FB, Kolli SK, ..., Adams JH 10.1038/s41598-024-51820-2
17041140 Developmental biology of sporozoite-host interactions in Plasmodium falciparummalaria: implications for vaccine design Garcia JE, Puentes A, Patarroyo ME 10.1128/CMR.00063-05
37703302 Preliminary characterization of Plasmodium vivax sporozoite antigens aspre-erythrocytic vaccine candidates Nicholas J, De SL, ..., Adams JH 10.1371/journal.pntd.0011598
27374339 The structure of sperm Izumo1 reveals unexpected similarities with Plasmodiuminvasion proteins Nishimura K, Han L, ..., Jovine L 10.1016/j.cub.2016.06.028