Genome location: Pf3D7_13_v3:1,673,239..1,677,299(+)
Genome classification: Core
Product Description: myosin A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0071944 | cell periphery |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0070258 | inner membrane pellicle complex |
| Component | GO:0016459 | myosin complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0020039 | pellicle |
| Component | GO:0048471 | perinuclear region of cytoplasm |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0031982 | vesicle |
| Function | GO:0005524 | ATP binding |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003779 | actin binding |
| Function | GO:0051015 | actin filament binding |
| Function | GO:0030898 | actin-dependent ATPase activity |
| Function | GO:0003774 | cytoskeletal motor activity |
| Function | GO:0000146 | microfilament motor activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0007015 | actin filament organization |
| Process | GO:0048870 | cell motility |
| Process | GO:0044409 | entry into host |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0030050 | vesicle transport along actin filament |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.01 | 0.71 |
| Trophozoite | expressed | 0.22 | 0.88 |
| Schizont | expressed | 0.62 | 0.78 |
| Gametocyte | expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1342600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -3.027 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1355700)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1355700)
Modification: Mutated | RMgm-4989
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDR3
"Best" AlphaFill ligand hit: BCN (bicine, Local RMSD=0.45) with 5T45 (Global RMSD=3.11)
No associated EC numbersOrthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100187
Most Similar Human Ortholog: J3KRL0
TM-align score: 0.91 | RMSD: 2.05
Seq Identity: 0.34 | Length: 421 / 818
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000128641 | myosin IB |
| ENSG00000136286 | myosin IG |
| ENSG00000142347 | myosin IF |
| ENSG00000157483 | myosin IE |
| ENSG00000166866 | myosin IA |
| ENSG00000174527 | myosin IH |
| ENSG00000176658 | myosin ID |
| ENSG00000197879 | myosin IC |
Protein Length: 818 | Molecular Weight (kDa): 92.277
PDB ID(s): 4AOM, 4MZJ, 4MZK, 4MZL, 4R1E, 6I7D, 6I7E, 6TU7, 6YCX, 6YCY, 6YCZ, 6ZN3, 7ALN, 8A12, 8CDQ
Isoelectric Point: 8.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001609 | Myosin head, motor domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR036044 | Class XIV myosin, motor domain |
| PFam | PF00063 | Myosin head, motor domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 31 | 38 | 42 |
| disruptive | 9 | 17 | 14 | 35 |
| missense | 9 | 17 | 14 | 30 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 83 | 29 | 29 |
| disruptive | 14 | 39 | 37 | 87 |
| missense | 12 | 29 | 32 | 59 |
PlasmoDB Total SNPs: 162
Non-coding: 140 | Synonymous: 12 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10749937 | A dibasic motif in the tail of a class XIV apicomplexan myosin is an essentialdeterminant of plasma membrane localization. | Hettmann C, Herm A, ..., Soldati D | 10.1091/mbc.11.4.1385 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16321976 | A conserved molecular motor drives cell invasion and gliding motility acrossmalaria life cycle stages and other apicomplexan parasites. | Baum J, Richard D, ..., Cowman AF | 10.1074/jbc.M509807200 |
| 16337961 | The MTIP-myosin A complex in blood stage malaria parasites. | Green JL, Martin SR, ..., Holder AA | 10.1016/j.jmb.2005.11.027 |
| 16547135 | Structure of the MTIP-MyoA complex, a key component of the malaria parasiteinvasion motor. | Bosch J, Turley S, ..., Hol WG | 10.1073/pnas.0510907103 |
| 20174678 | Interaction and dynamics of the Plasmodium falciparum MTIP-MyoA complex, a keycomponent of the invasion motor in the malaria parasite. | Thomas JC, Green JL, ..., Tate EW | 10.1039/b922093c |
| 21899701 | Stage-specific depletion of myosin A supports an essential role in motility ofmalarial ookinetes. | Siden-Kiamos I, Ganter M, ..., Matuschewski K | 10.1111/j.1462-5822.2011.01686.x |
| 22932904 | Regulation of the Plasmodium motor complex: phosphorylation of myosin Atail-interacting protein (MTIP) loosens its grip on MyoA. | Douse CH, Green JL, ..., Cota E | 10.1074/jbc.M112.379842 |
| 25802338 | The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location inInvasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain. | Yusuf NA, Green JL, ..., Holder AA | 10.1074/jbc.M115.637694 |
| 28759593 | Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodiumvivax salivary gland sporozoites. | Swearingen KE, Lindner SE, ..., Mikolajczak SA | https://pubmed.ncbi.nlm.nih.gov/28759593/ |
| 28893907 | Compositional and expression analyses of the glideosome during the Plasmodiumlife cycle reveal an additional myosin light chain required for maximum motility. | Green JL, Wall RJ, ..., Holder AA | 10.1074/jbc.M117.802769 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30599160 | Inner membrane complex 1l protein of Plasmodium falciparum links membrane lipidswith cytoskeletal element 'actin' and its associated motor 'myosin'. | Kumar V, Behl A, ..., Hora R | 10.1016/j.ijbiomac.2018.12.239 |
| 31337750 | Plasmodium myosin A drives parasite invasion by an atypical force generatingmechanism. | Robert-Paganin J, Robblee JP, ..., Houdusse A | 10.1038/s41467-019-11120-0 |
| 33046215 | Full-length Plasmodium falciparum myosin A and essential light chain Pf ELCstructures provide new anti-malarial targets | Moussaoui D, Robblee JP, ..., Houdusse A | 10.7554/eLife.60581 |
| 24632839 | The toxoplasma Acto-MyoA motor complex is important but not essential for glidingmotility and host cell invasion | Egarter S, Andenmatten N, ..., Meissner M | 10.1371/journal.pone.0091819 |
| 28100223 | Surface attachment, promoted by the actomyosin system of Toxoplasma gondii isimportant for efficient gliding motility and invasion | Whitelaw JA, Latorre-Barragan F, ..., Meissner M | 10.1186/s12915-016-0343-5 |
| 26760042 | The Conoid Associated Motor MyoH Is Indispensable for Toxoplasma gondii Entry andExit from Host Cells | Graindorge A, Frenal K, ..., Soldati-Favre D | 10.1371/journal.ppat.1005388 |
| 37155705 | MyosinA is a druggable target in the widespread protozoan parasite Toxoplasmagondii | Kelsen A, Kent RS, ..., Ward GE | 10.1371/journal.pbio.3002110 |
| 28972141 | Dissecting the molecular assembly of the Toxoplasma gondii MyoA motility complex | Powell CJ, Jenkins ML, ..., Boulanger MJ | 10.1074/jbc.M117.809632 |
| 31283102 | Systematic analysis of Plasmodium myosins reveals differential expression,localisation, and function in invasive and proliferative parasite stages | Wall RJ, Zeeshan M, ..., Tewari R | 10.1111/cmi.13082 |
| 33051581 | Structural role of essential light chains in the apicomplexan glideosome | Pazicky S, Dhamotharan K, ..., Low C | 10.1038/s42003-020-01283-8 |
| 30348763 | Structural and mechanistic insights into the function of the unconventional classXIV myosin MyoA from Toxoplasma gondii | Powell CJ, Ramaswamy R, ..., Boulanger MJ | 10.1073/pnas.1811167115 |
| 26374117 | Two Essential Light Chains Regulate the MyoA Lever Arm To Promote ToxoplasmaGliding Motility | Williams MJ, Alonso H, ..., Tonkin CJ | 10.1128/mBio.00845-15 |
| 25393004 | Plasticity between MyoC- and MyoA-glideosomes: an example of functionalcompensation in Toxoplasma gondii invasion | Frenal K, Marq JB, ..., Soldati-Favre D | 10.1371/journal.ppat.1004504 |
| 32383851 | Peptide Probes for Plasmodium falciparum MyoA Tail Interacting Protein (MTIP):Exploring the Druggability of the Malaria Parasite Motor Complex | Saunders CN, Cota E, Baum J, Tate EW | 10.1021/acschembio.0c00328 |
| 34011689 | Blocking Palmitoylation of Toxoplasma gondii Myosin Light Chain 1 DisruptsGlideosome Composition but Has Little Impact on Parasite Motility | Rompikuntal PK, Kent RS, ..., Ward GE | 10.1128/mSphere.00823-20 |
| 32432851 | Coupling Polar Adhesion with Traction, Spring, and Torque Forces AllowsHigh-Speed Helical Migration of the Protozoan Parasite Toxoplasma | Pavlou G, Touquet B, ..., Tardieux I | 10.1021/acsnano.0c01893 |
| 31874192 | Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetesand sporozoites | Siden-Kiamos I, Goosmann C, ..., Montagna GN | 10.1016/j.molbiopara.2019.111247 |
| 25367834 | Targeting a dynamic protein-protein interaction: fragment screening against themalaria myosin A motor complex | Douse CH, Vrielink N, ..., Tate EW | 10.1002/cmdc.201402357 |
| 23831369 | The compact conformation of the Plasmodium knowlesi myosin tail interactingprotein MTIP in complex with the C-terminal helix of myosin A | Turley S, Khamrui S, Bergman LW, Hol WG | 10.1016/j.molbiopara.2013.06.004 |
| 23831371 | The structure of the D3 domain of Plasmodium falciparum myosin tail interactingprotein MTIP in complex with a nanobody | Khamrui S, Turley S, ..., Hol WG | 10.1016/j.molbiopara.2013.06.003 |