Genome location: Pf3D7_13_v3:1,677,300..1,678,286(-)
Genome classification: Core
Product Description: DNA-directed RNA polymerases I, II, and III subunit RPABC4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Function | GO:0003700 | DNA-binding transcription factor activity |
| Process | GO:0006351 | transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.07 | 0.96 |
| Schizont | not expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1342700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: N/A
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1355800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDR2
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103748
Most Similar Human Ortholog: P53803
TM-align score: 0.73 | RMSD: 1.84
Seq Identity: 0.39 | Length: 49 / 58
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000147669 | RNA polymerase II, I and III subunit K |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 79
Non-coding: 77 | Synonymous: 1 | Nonsynonymous: 0 | Stop Codon: 1
Protein Length: 58 | Molecular Weight (kDa): 6.746
UniProt ID(s): Q8IDR2
PDB ID(s): None
Isoelectric Point: 8.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006591 | RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 |
| InterPro | IPR029040 | RNA polymerase subunit RPABC4/transcription elongation factor Spt4 |
| InterPro | IPR039747 | DNA-directed RNA polymerases I, II, and III subunit RPABC4 |
| PFam | PF03604 | RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 |
| Superfamily | SSF63393 | RNA polymerase subunit RPABC4/transcription elongation factor Spt4 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11168400 | Intracellular contents and assembly states of all 12 subunits of the RNApolymerase II in the fission yeast Schizosaccharomyces pombe | Kimura M, Sakurai H, Ishihama A | 10.1046/j.1432-1327.2001.01911.x |
| 30395243 | Genome-wide transcriptional response to altered levels of the Rpb7 subunit of RNApolymerase II identifies its role in DNA damage response in Schizosaccharomycespombe | Kumar D, Sharma N | 10.1093/femsyr/foy118 |
| 11168594 | Transcription organization and mRNA levels of the genes for all 12 subunits ofthe fission yeast RNA polymerase II | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2001.00394.x |
| 15743411 | Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functionalchanges in RNA polymerase II at transcription initiation | Hayashi K, Watanabe T, ..., Ohkuma Y | 10.1111/j.1365-2443.2005.00833.x |
| 16962183 | Biochemical characterization of Trypanosoma brucei RNA polymerase II | Das A, Li H, Liu T, Bellofatto V | 10.1016/j.molbiopara.2006.08.002 |
| 35618649 | Absence of the Rpb9 subunit of RNA polymerase II reduces the chronological lifespan in fission yeast | Bhardwaj V, Sharma N | 10.1002/jobm.202200036 |
| 14698245 | Heterologous overexpression and purification of four common subunits of nuclearRNA polymerases I, II and III of Schizosaccharomyces pombe | Proshkin SA, Shpakovski GV | 10.1016/j.jchromb.2003.09.008 |
| 11918671 | Level of the RNA polymerase II in the fission yeast stays constant butphosphorylation of its carboxyl terminal domain varies depending on the phase andrate of cell growth | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2002.00522.x |
| 18467854 | A novel approach to investigating protein/protein interactions and theirfunctions by TAP-tagged yeast strains and its application to examine yeasttranscription machinery | Jung J, Ahn YJ, Kang LW | https://pubmed.ncbi.nlm.nih.gov/18467854/ |
| 16972065 | The fission yeast Rpb4 subunit of RNA polymerase II plays a specialized role incell separation | Sharma N, Marguerat S, ..., Bahler J | 10.1007/s00438-006-0161-5 |