Genome location: Pf3D7_13_v3:1,678,802..1,682,001(+)
Genome classification: Core
Product Description: phosphoenolpyruvate carboxykinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.1.1.49 (Phosphoenolpyruvate carboxykinase (ATP))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004612 | phosphoenolpyruvate carboxykinase (ATP) activity |
| Process | GO:0006094 | gluconeogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.35 | 0.80 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1342800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.265 | MFS: -2.655 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1355900)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1355900)
Modification: Disrupted | RMgm-4082
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDR1
"Best" AlphaFill ligand hit: PYR (pyruvic acid, Local RMSD=0.26) with 1AQ2 (Global RMSD=1.99)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.1.1.49 | phosphoenolpyruvate carboxykinase (ATP) | ATPKClNEMIDPNADHDTNBazideMnCl2malateoxalate(NH4)2SO4iodoacetamide... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 29 | 23 | 39 |
| disruptive | 0 | 14 | 14 | 38 |
| missense | 0 | 14 | 14 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 91 | 26 | 27 |
| disruptive | 4 | 46 | 26 | 85 |
| missense | 2 | 37 | 25 | 65 |
PlasmoDB Total SNPs: 112
Non-coding: 94 | Synonymous: 14 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 583 | Molecular Weight (kDa): 66.209
PDB ID(s): None
Isoelectric Point: 6.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001272 | Phosphoenolpyruvate carboxykinase, ATP-utilising |
| InterPro | IPR008210 | Phosphoenolpyruvate carboxykinase, N-terminal |
| InterPro | IPR015994 | Phosphoenolpyruvate carboxykinase (ATP), conserved site |
| PFam | PF01293 | Phosphoenolpyruvate carboxykinase, ATP-utilising |
| Superfamily | SSF53795 | Phosphoenolpyruvate carboxykinase, N-terminal |
| Superfamily | SSF68923 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 28726641 | A plant/fungal-type phosphoenolpyruvate carboxykinase located in the parasitemitochondrion ensures glucose-independent survival of Toxoplasma gondii. | Nitzsche R, Gunay-Esiyok O, ..., Gupta N | 10.1074/jbc.M117.802702 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |