Genome location: Pf3D7_13_v3:1,695,462..1,699,267(+)
Genome classification: Core
Product Description: phosphoethanolamine N-methyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.103 (Phosphoethanolamine N-methyltransferase);2.1.1.141 (Jasmonate O-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Function | GO:0003723 | RNA binding |
| Function | GO:0000234 | phosphoethanolamine N-methyltransferase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006656 | phosphatidylcholine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.59 | 0.36 |
| Trophozoite | expressed | 2.93 | 0.13 |
| Schizont | expressed | 1.97 | 0.30 |
| Gametocyte | possibly expressed | 0.74 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1343000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -3.113 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDQ9
"Best" AlphaFill ligand hit: OPE (phosphoric acid mono-(2-amino-ethyl) ester, Local RMSD=0.17) with 3UJA (Global RMSD=0.20)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 195
Non-coding: 179 | Synonymous: 10 | Nonsynonymous: 5 | Stop Codon: 1
Protein Length: 266 | Molecular Weight (kDa): 31.043
PDB ID(s): None
Isoelectric Point: 5.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| InterPro | IPR041698 | Methyltransferase domain 25 |
| PFam | PF13649 | Methyltransferase domain 25 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15073329 | A pathway for phosphatidylcholine biosynthesis in Plasmodium falciparum involvingphosphoethanolamine methylation. | Pessi G, Kociubinski G, Mamoun CB | 10.1073/pnas.0307742101 |
| 15664981 | In vivo evidence for the specificity of Plasmodium falciparum phosphoethanolaminemethyltransferase and its coupling to the Kennedy pathway. | Pessi G, Choi JY, ..., Mamoun CB | 10.1074/jbc.M414626200 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16704982 | Localization of the phosphoethanolamine methyltransferase of the human malariaparasite Plasmodium falciparum to the Golgi apparatus. | Witola WH, Pessi G, ..., Mamoun CB | 10.1074/jbc.M603260200 |
| 17644653 | Choline induces transcriptional repression and proteasomal degradation of themalarial phosphoethanolamine methyltransferase. | Witola WH, Ben Mamoun C | 10.1128/EC.00229-07 |
| 18178564 | Biochemical and genetic analysis of the phosphoethanolamine methyltransferase ofthe human malaria parasite Plasmodium falciparum. | Reynolds JM, Takebe S, ..., Mamoun CB | 10.1074/jbc.M709869200 |
| 18694927 | Disruption of the Plasmodium falciparum PfPMT gene results in a complete loss ofphosphatidylcholine biosynthesis via theserine-decarboxylase-phosphoethanolamine-methyltransferase pathway and severegrowth and survival defects. | Witola WH, El Bissati K, ..., Mamoun CB | 10.1074/jbc.M804360200 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 22117061 | Structure and reaction mechanism of phosphoethanolamine methyltransferase fromthe malaria parasite Plasmodium falciparum: an antiparasitic drug target. | Lee SG, Kim Y, ..., Jez JM | 10.1074/jbc.M111.315267 |
| 25288796 | An alternative mechanism for the methylation of phosphoethanolamine catalyzed byPlasmodium falciparum phosphoethanolamine methyltransferase. | Saen-Oon S, Lee SG, Jez JM, Guallar V | 10.1074/jbc.M114.611319 |
| 26988354 | Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 inPlasmodium falciparum. | Hollin T, De Witte C, ..., Khalife J | 10.1186/s12864-016-2571-z |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 38258560 | Effect of ATG8 or SAC1 deficiency on the cell proliferation and lifespan of thelong-lived PMT1 deficiency yeast cells | Cui H, Cui X, ..., Liu X | 10.1093/femsle/fnad121 |