PF3D7_1343200

Genome location: Pf3D7_13_v3:1,703,564..1,707,279(+)

Genome classification: Core

Function and Localization

Product Description: tRNA guanosine-2'-O-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.34 (tRNA (guanosine(18)-2'-O)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0008168	methyltransferase activity
Function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.19	0.91
Trophozoite	expressed	0.40	0.76
Schizont	expressed	0.44	0.73
Gametocyte	expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1343200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.659 | #Insertions: 2

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1356200)

Relative Growth Rate: 0.79 ± 0.15
Confidence: 5.19

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1356200)

Modification: Disrupted | RMgm-3672

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDQ7

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=7.01) with 7QW5 (Global RMSD=12.32)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.34	tRNA (guanosine18-2'-O)-methyltransferase	PolyamineDELTAD-armS-adenosyl-L-homocysteine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102168

Most Similar Human Ortholog: K7ENP1

TM-align score: 0.84 | RMSD: 2.49

Seq Identity: 0.29 | Length: 220 / 517

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000066651	tRNA methyltransferase 11 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	31	14	29
disruptive	9	60	43	69
missense	8	56	39	63

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	50	27	35
disruptive	25	152	81	171
missense	19	129	69	99

PlasmoDB Total SNPs: 193

Non-coding: 153 | Synonymous: 27 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 517 | Molecular Weight (kDa): 60.462

UniProt ID(s): Q8IDQ7

PDB ID(s): None

Isoelectric Point: 8.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000241	Putative RNA methylase domain
InterPro	IPR002052	DNA methylase, N-6 adenine-specific, conserved site
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF01170	Putative RNA methylase domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase