Genome location: Pf3D7_13_v3:1,714,271..1,719,409(+)
Genome classification: Core
Product Description: DNA repair protein RAD5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0003678 | DNA helicase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.41 | 0.80 |
| Trophozoite | expressed | 0.67 | 0.63 |
| Schizont | possibly expressed | 0.41 | 0.76 |
| Gametocyte | not expressed | 0.79 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1343400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.147 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1356400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1356400)
Modification: Disrupted | RMgm-3674
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDQ5
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.17) with 3MWY (Global RMSD=6.63)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101296
Most Similar Human Ortholog: H7C5K0
TM-align score: 0.76 | RMSD: 2.51
Seq Identity: 0.37 | Length: 346 / 1452
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000071794 | helicase like transcription factor |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 93 | 49 | 99 |
| disruptive | 15 | 144 | 100 | 226 |
| missense | 15 | 140 | 97 | 207 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 38 | 180 | 60 | 108 |
| disruptive | 46 | 331 | 155 | 314 |
| missense | 35 | 293 | 135 | 229 |
PlasmoDB Total SNPs: 168
Non-coding: 85 | Synonymous: 49 | Nonsynonymous: 34 | Stop Codon: 0
Protein Length: 1452 | Molecular Weight (kDa): 169.76
UniProt ID(s): Q8IDQ5
PDB ID(s): None
Isoelectric Point: 8.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000330 | SNF2-related, N-terminal domain |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR001841 | Zinc finger, RING-type |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR017907 | Zinc finger, RING-type, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00176 | SNF2-related, N-terminal domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF57850 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 37054652 | Helicase activities of Rad5 and Rrm3 genetically interact in the prevention ofrecombinogenic DNA lesions in Saccharomyces cerevisiae | Muellner J, Schmidt KH | 10.1016/j.dnarep.2023.103488 |
| 37594077 | Two independent DNA repair pathways cause mutagenesis in template switchingdeficient Saccharomyces cerevisiae | Jiang YK, Medley EA, Brown GW | 10.1093/genetics/iyad153 |
| 36861970 | Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the RegulatedRecruitment of Srs2 Helicase | Fan L, Zhang W, ..., Xiao W | 10.1128/mbio.00315-23 |