PF3D7_1343600

Genome location: Pf3D7_13_v3:1,721,704..1,724,056(-)

Genome classification: Core

Function and Localization

Product Description: UDP-N-acetylglucosamine pyrophosphorylase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003977	UDP-N-acetylglucosamine diphosphorylase activity
Process	GO:0006048	UDP-N-acetylglucosamine biosynthetic process
Process	GO:0006047	UDP-N-acetylglucosamine metabolic process
Process	GO:0009226	nucleotide-sugar biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.17	0.91
Trophozoite	expressed	0.37	0.77
Schizont	possibly expressed	0.06	0.96
Gametocyte	expressed	0.23	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1343600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.129 | MFS: -3.188 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1356600)

Relative Growth Rate: 0.81 ± 0.07
Confidence: 6.73

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1356600)

Modification: Disrupted | RMgm-4617

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDQ3

"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.08) with 6G9V (Global RMSD=3.34)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.23	UDP-N-acetylglucosamine diphosphorylase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101381

Most Similar Human Ortholog: Q3KQV9

TM-align score: 0.85 | RMSD: 2.66

Seq Identity: 0.28 | Length: 466 / 593

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000117143	UDP-N-acetylglucosamine pyrophosphorylase 1
ENSG00000197355	UDP-N-acetylglucosamine pyrophosphorylase 1 like 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	28	13	38
disruptive	16	94	65	122
missense	13	93	61	115

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	55	31	47
disruptive	29	205	97	174
missense	23	189	85	137

PlasmoDB Total SNPs: 80

Non-coding: 31 | Synonymous: 25 | Nonsynonymous: 24 | Stop Codon: 0

Protein Information

Protein Length: 593 | Molecular Weight (kDa): 69.984

UniProt ID(s): Q8IDQ3

PDB ID(s): None

Isoelectric Point: 8.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002618	UDPGP family
InterPro	IPR029044	Nucleotide-diphospho-sugar transferases
InterPro	IPR039741	UDP-sugar pyrophosphorylase
PFam	PF01704	UDPGP family
Superfamily	SSF53448	Nucleotide-diphospho-sugar transferases