PF3D7_1343900 (PRPF4)

Genome location: Pf3D7_13_v3:1,755,697..1,758,708(+)

Genome classification: Core

Function and Localization

Product Description: U4/U6 small nuclear ribonucleoprotein PRP4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0046540	U4/U6 x U5 tri-snRNP complex
Function	GO:0030621	U4 snRNA binding
Function	GO:0017070	U6 snRNA binding
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.31	0.85
Trophozoite	no data	0.50	0.71
Schizont	no data	0.25	0.83
Gametocyte	no data	0.32	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1343900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.131 | MFS: -2.85 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1356900)

Relative Growth Rate: 0.21 ± 0.14
Confidence: 5.36

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5G7

"Best" AlphaFill ligand hit: SIW (keggin (sta), Local RMSD=0.05) with 6Y7O (Global RMSD=2.49)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102752

Most Similar Human Ortholog: O43172

TM-align score: 0.68 | RMSD: 3.15

Seq Identity: 0.28 | Length: 390 / 648

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136875	pre-mRNA processing factor 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	35	23	32
disruptive	20	85	55	120
missense	8	79	53	109

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	63	23	51
disruptive	33	177	91	153
missense	18	153	82	121

PlasmoDB Total SNPs: 128

Non-coding: 67 | Synonymous: 38 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 648 | Molecular Weight (kDa): 76.19

UniProt ID(s): C0H5G7

PDB ID(s): None

Isoelectric Point: 5.69

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR014906	Pre-mRNA processing factor 4 (PRP4)-like
InterPro	IPR019775	WD40 repeat, conserved site
InterPro	IPR036285	PRP4-like superfamily
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
PFam	PF08799	Pre-mRNA processing factor 4 (PRP4)-like
Superfamily	SSF158230	PRP4-like superfamily
Superfamily	SSF50978	WD40-repeat-containing domain superfamily