PF3D7_1344000

Genome location: Pf3D7_13_v3:1,758,930..1,762,648(+)

Genome classification: Core

Function and Localization

Product Description: aminomethyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.2.10 (Aminomethyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0004047	aminomethyltransferase activity
Process	GO:0019464	glycine decarboxylation via glycine cleavage system

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.09	0.95
Trophozoite	no data	0.37	0.78
Schizont	no data	0.06	0.96
Gametocyte	no data	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1344000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.172 | MFS: -2.765 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5G8

"Best" AlphaFill ligand hit: RED (dihydrolipoic acid, Local RMSD=0.19) with 1WOR (Global RMSD=2.46)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.2.10	aminomethyltransferase	iodoacetamideN-ethylmaleimide

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_179354

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	75	23	45
disruptive	20	113	56	183
missense	19	111	55	171

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	104	42	58
disruptive	45	220	107	195
missense	37	209	100	160

PlasmoDB Total SNPs: 171

Non-coding: 103 | Synonymous: 47 | Nonsynonymous: 20 | Stop Codon: 1

Protein Information

Protein Length: 841 | Molecular Weight (kDa): 98.779

UniProt ID(s): C0H5G8

PDB ID(s): None

Isoelectric Point: 8.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006222	Aminomethyltransferase, folate-binding domain
InterPro	IPR013977	Glycine cleavage T-protein, C-terminal barrel domain
InterPro	IPR029043	Glycine cleavage T-protein/YgfZ, C-terminal
PFam	PF01571	Aminomethyltransferase, folate-binding domain
PFam	PF08669	Glycine cleavage T-protein, C-terminal barrel domain
Superfamily	SSF101790	Glycine cleavage T-protein/YgfZ, C-terminal
Superfamily	SSF103025